| Title: |
Atomic Force Microscopy Reveals Differences In Mechanical Properties Linked To Cortical Structure In Mouse And Human Oocytes |
| Authors: |
Bulteau, Rose; Barbier, Lucie; Lamour, Guillaume; Lemseffer, Yassir; Verlhac, Marie‐hélène; Tessandier, Nicolas; Labrune, Elsa; Lenz, Martin; Terret, Marie‐emilie; Campillo, Clément |
| Contributors: |
Centre interdisciplinaire de recherche en biologie (CIRB); Labex MemoLife; École normale supérieure - Paris (ENS-PSL); Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris); Université Paris Sciences et Lettres (PSL)-École normale supérieure - Paris (ENS-PSL); Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS); Laboratoire Analyse, Modélisation et Matériaux pour la Biologie et l'Environnement (LAMBE - UMR8587); Université d'Évry-Val-d'Essonne (UEVE)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-CY Cergy Paris Université (CY); Institut cellule souche et cerveau / Stem Cell and Brain Research Institute (SBRI); Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Laboratoire de Physique Théorique et Modèles Statistiques (LPTMS); Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS); Institut universitaire de France (IUF); Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.); DIM ELICIT du Conseil regional d'Ile de France (DIM ELICIT-AAP-2020); Biomedical Engineering seed grant program; FRM ECO- Contrat doctoral program (ECO202206015524); Fondation Bettencourt-Schueller; ANR-17-CONV-0005,Q-LIFE,Institut Q-LIFE(2017); ANR-24-CE13-5691,CoPOcyte,Tension corticale, polarisation et potentiel de développement des ovocytes de mammifères(2024); ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010); ANR-10-LABX-0054,MEMOLIFE,Memory in living systems: an integrated approach(2010); ANR-10-IDEX-0001,PSL,Paris Sciences et Lettres(2010) |
| Source: |
ISSN: 1613-6810. |
| Publisher Information: |
CCSD; Wiley-VCH Verlag |
| Publication Year: |
2025 |
| Collection: |
Inserm: HAL (Institut national de la santé et de la recherche médicale) |
| Subject Terms: |
oocytes; elastic modulus; cortical tension; biomechanics; atomic force microscopy; actomyosin cortex; [PHYS]Physics [physics] |
| Description: |
International audience ; Cell mechanical properties regulate biological processes such as oocyte development. Cortical tension is regulated via actomyosin cortex remodeling to ensure optimal oocyte quality. However, the evolution of other mechanical parameters and their relationship with cortex structure remain poorly understood in mammalian oocytes. In this work, a methodology combining multiple mechanical parameters measured through Atomic Force Microscopy is proposed to investigate the relationship between oocyte mechanical properties and cortex organization. By studying mouse oocytes at various stages of development, along with engineered ones with specific cortex organization, it is demonstrated that a thin actin cortex corresponds to stiff oocytes while a thick one is associated with softer oocytes. It is further revealed that maternal age, a critical factor for fertility, affects mouse oocytes mechanics, correlating with alterations in their cortex structure. Finally, it is shown that the evolution of mechanical properties differs between human and mouse oocyte development, highlighting species-specific differences in cortex organization. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1002/smll.202500221 |
| Availability: |
https://hal.science/hal-05030230; https://hal.science/hal-05030230v1/document; https://hal.science/hal-05030230v1/file/Bulteau%202025.pdf; https://doi.org/10.1002/smll.202500221 |
| Rights: |
https://creativecommons.org/licenses/by-nc-nd/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.3E4CF33A |
| Database: |
BASE |