| Title: |
In vitro Effects of Calcium, Magnesium, Mercury and Copper Ions on Neuraminidase of a Nigerian Newcastle Disease Virus Strains |
| Authors: |
Sunday Blessing Oladele; King Akpofure; Nelson Esievo; Paul Abdu; Andrew Jonathan Nok |
| Contributors: |
The Pennsylvania State University CiteSeerX Archives |
| Source: |
http://www.pjbs.org/ijps/fin1053.pdf. |
| Collection: |
CiteSeerX |
| Description: |
This study was carried out to determine the effects of cations on neuraminidase during NDV infections in poultry. A total of two hundred samples from dialyzed neuraminidase of NDV Kudu 113 strain were used for the experiment. Fifty samples each were used on four divalent cations, namely calcium (Ca),2+ magnesium (Mg), mercury (Hg) and copper (Cu) ions. Neuraminidase activity of NDV Kudu 113 strain2+ 2+ 2+ was determined in vitro in the presence of these cations, tested as their chloride form by periodate thiobarbituric acid assay method. Neuraminidase activity was also determined in the control samples that were without cations. Out of the four divalent cations examined, Hg induced the highest neuraminidase2+ activity of 75 % at the concentration of 0.24M. Magnesium and Ca ions also increased the activity of2+ neuraminidase of NDV Kudu 113 strain in vitro. On the other hand, Cu significantly inhibited the activity of2+ this enzyme by 69.23 % at the concentration of 0.47M. It was concluded that Ca, Mg and Hg induced2+ 2+ 2+ increase in the activity of neuraminidase of NDV Kudu 113 strain, while Cu inhibited its activity in vitro.2+ Further studies should be carried out on the roles of these cations in Newcastle disease (ND) and the possible use of Cu in the future as inhibitor of the activity of neuraminidase of NDV in vivo, so as to reduce2+ the menance of ND in poultry. Key words: Cation, effect, neuraminidase and newcastle disease virus |
| Document Type: |
text |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.610.1093; http://www.pjbs.org/ijps/fin1053.pdf |
| Availability: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.610.1093; http://www.pjbs.org/ijps/fin1053.pdf |
| Rights: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| Accession Number: |
edsbas.3F2B6743 |
| Database: |
BASE |