| Title: |
Preparative isolation of apple Flavan-3-ols by pH-zone-refining centrifugal partition chromatography combined with reversed-phase liquid chromatography |
| Authors: |
Guilois Dubois, Sophie; Guyot, Sylvain; Poupard, Pascal |
| Contributors: |
Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA); Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Insitut Français des Productions Cidricoles (IFPC) |
| Source: |
ICP2021, XXX International Conference on Polyphenol ; https://hal.inrae.fr/hal-04684202 ; ICP2021, XXX International Conference on Polyphenol, Jul 2021, Turku, Finland |
| Publisher Information: |
CCSD |
| Publication Year: |
2021 |
| Collection: |
Institut National de la Recherche Agronomique: ProdINRA |
| Subject Terms: |
Tanin; Fractionation Procedures; Cider apple; Polyphenol; [CHIM]Chemical Sciences; [SDV.IDA]Life Sciences [q-bio]/Food engineering |
| Subject Geographic: |
Turku; Finland |
| Description: |
International audience ; MAIN CONCLUSION A new procedure combining centrifugal partition chromatography (CPC) and preparative reversed phase chromatography (Prep-RP-HPLC) allowed recovering a highly purified flavan-3-ol fraction from a crude cider apple polyphenol extract. Our methodological development demonstrated the relevance of using CPC combined with a pH-displacement mode to efficiently discard hydroxycinnamic acid esters on a preparative scale. This step was followed by Prep-RP-HPLC to remove dihydrochalcones and flavonols. INTRODUCTION Cider apples are naturally rich in phenolic compounds that are divided in several classes including flavan-3-ols, hydroxycinnamic acid esters (HCA), flavonols (FO), and dihydrochalcones (DHC). Among them, flavanols (i.e. catechin monomers and procyanidin oligomers) are of particular interest exhibiting numerous properties in food matrix such as tanning properties, organoleptic (bitterness and astringency), nutritional effects, and biological activity (antimicrobial, antioxidant, etc.). In general, their fractionation, mainly based on liquid chromatography on solid phases, is tedious, time-consuming, and solvent-wasting, with the risk of losing a part of the tannins through irreversible adsorption. Moreover, reversed-phase HPLC with a classical gradient using a water/organic solvent mixture elutes apple procyanidins and catechin monomers throughout the chromatogram without any clear separation from HCA, FO, or DHC phenolic classes. In this study, a gentle strategy was developed to recover a highly purified flavan-3-ol fraction. Firstly, pH-zone-refining centrifugal partition chromatography (pH-ZRCPC) was optimized to discard HCA using their ionisation property. Finally, preparative reversed-phase liquid chromatography (Prep-RPLC) was used to remove the others unwanted compounds (DHC and FO). MATERIALS & METHODS A crude extract from a polyphenol-rich French cider apple cultivar was obtained from a juice according to the method described by Millet et al. [1]. Biphasic ... |
| Document Type: |
conference object; still image |
| Language: |
English |
| Availability: |
https://hal.inrae.fr/hal-04684202; https://hal.inrae.fr/hal-04684202v1/document; https://hal.inrae.fr/hal-04684202v1/file/Poster%20ICP%202021_V5_20210628.pdf |
| Rights: |
https://about.hal.science/hal-authorisation-v1/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.41B5B453 |
| Database: |
BASE |