| Title: |
A fluorescent photoaffinity probe for formyl peptide receptor 1 labelling in living cells |
| Authors: |
Field, DH; White, JS; Warriner, S; Wright, MH |
| Publisher Information: |
Royal Society of Chemistry |
| Publication Year: |
2023 |
| Collection: |
White Rose Research Online (Universities of Leeds, Sheffield & York) |
| Description: |
Fluorescent ligands for G-protein coupled receptors (GPCRs) are valuable tools for studying the expression, pharmacology and modulation of these therapeutically important proteins in living cells. Here we report a fluorescent photoaffinity probe for Formyl peptide receptor 1 (FPR1), a critical component of the innate immune response to bacterial infection and a promising target in inflammatory diseases. We demonstrate that the probe binds and covalently crosslinks to FPR1 with good specificity at nanomolar concentrations in living cells and is a useful tool for visualisation and characterisation of this receptor. |
| Document Type: |
article in journal/newspaper |
| File Description: |
text |
| Language: |
English |
| ISSN: |
2633-0679 |
| Relation: |
https://eprints.whiterose.ac.uk/id/eprint/195435/26/A%20fluorescent%20photoaffinity%20probe%20for%20formyl.pdf; Field, DH, White, JS, Warriner, S orcid.org/0000-0002-7589-6011 et al. (1 more author) (2023) A fluorescent photoaffinity probe for formyl peptide receptor 1 labelling in living cells. RSC Chemical Biology, 4 (3). pp. 216-222. ISSN: 2633-0679 |
| Availability: |
https://eprints.whiterose.ac.uk/id/eprint/195435/; https://eprints.whiterose.ac.uk/id/eprint/195435/26/A%20fluorescent%20photoaffinity%20probe%20for%20formyl.pdf |
| Rights: |
cc_by_3 |
| Accession Number: |
edsbas.42E20F3F |
| Database: |
BASE |