| Title: |
3 Administration of a DNA immunostimulant does not mitigate bovine herpesvirus-1 recrudescence in dexamethasone challenged beef cattle |
| Authors: |
Seiver, Hannah A; Samuelson, Kendall L; Posey, Richard D; Tomczak, Dexter J; Smock, Taylor M; Richeson, John T |
| Source: |
Journal of Animal Science ; volume 97, issue Supplement_1, page 16-17 ; ISSN 0021-8812 1525-3163 |
| Publisher Information: |
Oxford University Press (OUP) |
| Publication Year: |
2019 |
| Description: |
The study objective was to determine the effect of a DNA immunostimulant on recrudescence of bovine herpesvirus-1 (BHV-1) after dexamethasone challenge in beef cattle. It was hypothesized that the DNA immunostimulant would mitigate stress-induced immunosuppression; thereby, reducing the incidence of BHV-1 recrudescence. Steers (n = 10) and heifers (n = 10; initial BW = 489 kg ± 57 kg) were stratified by pre-existing BHV-1 antibody titer, sex and initial BW and randomly assigned to treatment (n = 4 pens/treatment; 2 or 3 animals/pen). All calves were administered 40 mg of dexamethasone i.v. at 0600 h from d 0 to 2, 166-d subsequent to BHV-1 challenge with 1.0 × 108 plaque-forming units per nostril. On d 1, calves were administered treatments consisting of 2 mL i.m. of DNA immunostimulant (Zelnate; ZEL) or sterile saline (CON). Once daily (0600) from d 0 to 12, a whole blood was obtained via jugular venipuncture for complete blood count (CBC) analysis and nasal swabs were collected to determine BHV-1 prevalence via virus isolation testing. A repeated measures mixed model was used to test the effect of treatment, day and their interaction for CBC variables. There was a treatment × day interaction for eosinophils (P = 0.02) and percent eosinophils (P = 0.03). Eosinophils were greater (P < 0.01) for ZEL on d 3 and 6 post-dexamethasone challenge. On d 11 and 12, eosinophils for CON rebounded such that their concentration was greater than ZEL (P < 0.01). Lymphocytes, neutrophil and monocyte concentration did not differ (P ≥ 0.44); however, a day effect (P ≤ 0.01) existed such that each variable increased transiently after dexamethasone challenge. All cattle had BHV-1 present in a nasal swab sample on at least one sample day, with prevalence of BHV-1 in nasal swab samples being greatest on d 5 (80% positive; P = 0.01). However, no treatment differences were detected for BHV-1 prevalence in this study. The DNA immunostimulant altered eosinophil concentrations but did not mitigate BHV-1 recrudesce after ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1093/jas/skz053.037 |
| Availability: |
https://doi.org/10.1093/jas/skz053.037; http://academic.oup.com/jas/article-pdf/97/Supplement_1/16/29014346/skz053.037.pdf |
| Rights: |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| Accession Number: |
edsbas.43E3A04E |
| Database: |
BASE |