The Fission Yeast S-Phase Cyclin Cig2 Can Drive Mitosis [preprint]
| Title: | The Fission Yeast S-Phase Cyclin Cig2 Can Drive Mitosis [preprint] |
|---|---|
| Authors: | Magner, Mira; Keifenheim, Daniel L.; Rhind, Nicholas |
| Contributors: | Department of Biochemistry and Molecular Pharmacology |
| Source: | bioRxiv |
| Publication Year: | 2022 |
| Collection: | University of Massachusetts, Medical School: eScholarship@UMMS |
| Subject Terms: | fission yeast; Schizosaccharomyces pombe; Cdc13; cyclin; cyclin-dependent kinase; Cig2; cell biology; Amino Acids; Peptides; and Proteins; Cells; Enzymes and Coenzymes |
| Description: | Commitment to mitosis is regulated by cyclin-dependent kinase (CDK) activity. In the fission yeast Schizosaccharomyces pombe, the major B-type cyclin, Cdc13, is necessary and sufficient to drive mitotic entry. Furthermore, Cdc13 is also sufficient to drive S phase, demonstrating that a single cyclin can regulate alternating rounds of replication and mitosis and providing the foundation of the quantitative model of CDK function. It has been assumed that Cig2, a B-type cyclin expressed only during S-phase and incapable of driving mitosis in wild-type cells, was specialized for S-phase regulation. Here, we show that Cig2 is capable of driving mitosis. Cig2/CDK activity drives mitotic catastrophe -- lethal mitosis in inviably small cells -- in cells that lack CDK inhibition by tyrosine-phosphorylation. Moreover, Cig2/CDK can drive mitosis in the absence of Cdc13/CDK activity. These results demonstrate that in fission yeast, not only can the presumptive M-phase cyclin drive S phase, but the presumptive S-phase cyclin can drive M phase, further supporting the quantitative model of CDK function. Furthermore, these results provide an explanation, previously proposed on the basis of computation analyses, for the surprising observation that cells expressing a single-chain Cdc13-Cdc2 CDK do not require Y15 phosphorylation for viability. Their viability is due to the fact that in such cells, which lack Cig2/CDK complexes, Cdc13/CDK activity is unable to drive mitotic catastrophe. |
| Document Type: | report |
| File Description: | application/pdf |
| Language: | English |
| Relation: | Now published in Genetics doi:10.1093/genetics/iyaa002; bioRxiv 213330; doi: https://doi.org/10.1101/213330. Link to preprint on bioRxiv service.; http://hdl.handle.net/20.500.14038/29294; https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2527&context=faculty_pubs&unstamped=1; https://escholarship.umassmed.edu/faculty_pubs/1521; faculty_pubs/1521 |
| DOI: | 10.1101/213330 |
| Availability: | https://doi.org/10.1101/213330; https://hdl.handle.net/20.500.14038/29294; https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2527&context=faculty_pubs&unstamped=1; https://escholarship.umassmed.edu/faculty_pubs/1521 |
| Rights: | The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC 4.0 International license. ; http://creativecommons.org/licenses/by-nc/4.0/ |
| Accession Number: | edsbas.442671AB |
| Database: | BASE |