| Title: |
CYP450 core involvement in multiple resistance strains of Aedes aegypti from French Guiana highlighted by proteomics, molecular and biochemical studies |
| Authors: |
Epelboin, Yanouk; Wang, Lanjiao; Giai Gianetto, Quentin; Choumet, Valérie; Gaborit, Pascal; Issaly, Jean; Guidez, Amandine; Douché, Thibaut; Chaze, Thibault; Matondo, Mariette, I; Dusfour, Isabelle |
| Contributors: |
Entomologie médicale Cayenne, Guyane française; Institut Pasteur de la Guyane; Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur); Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology (UTechS MSBio); Institut Pasteur Paris (IP)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS); Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB; Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS); Plateforme de Protéomique / Proteomics platform; Institut Pasteur Paris (IP)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur Paris (IP)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS); Environnement et Risques infectieux - Environment and Infectious Risks (ERI); Institut Pasteur Paris (IP); Département de Santé Globale - Department Global Health; This study was funded by the European Funds for Regional Development and CNES under FEDER “CONTROLE”, project n° Synergie: GY0010695 awarded to YE, LW, PG, JI, AG, and ID. This study was also funded by "an Investissement d’Avenir grant of the Agence Nationale de la Recherche" (CEBA: ANR-10-LABX-25-01) in which the Institut Pasteur de la Guyane is partner awarded to YE, LW, PG, JI, AG, and ID.; ANR-10-LABX-0025,CEBA,CEnter of the study of Biodiversity in Amazonia(2010) |
| Source: |
ISSN: 1932-6203. |
| Publisher Information: |
CCSD; Public Library of Science |
| Publication Year: |
2021 |
| Collection: |
Réseau International des Instituts Pasteur, Paris: HAL-RIIP |
| Subject Terms: |
[SDV.BBM]Life Sciences [q-bio]/Biochemistry; Molecular Biology |
| Description: |
International audience ; Insecticide resistance is a worldwide threat for vector control around the world, and Aedes aegypti, the main vector of several arboviruses, is a particular concern. To better understand the mechanisms of resistance, four isofemale strains originally from French Guiana were isolated and analysed using combined approaches. The activity of detoxification enzymes involved in insecticide resistance was assayed, and mutations located at positions 1016 and 1534 of the sodium voltage-gated channel gene, which have been associated with pyrethroid resistance in Aedes aegypti populations in Latin America, were monitored. Resistance to other insecticide families (organophosphates and carbamates) was evaluated. A large-scale proteomic analysis was performed to identify proteins involved in insecticide resistance. Our results revealed a metabolic resistance and resistance associated with a mutation of the sodium voltage-gated channel gene at position 1016. Metabolic resistance was mediated through an increase of esterase activity in most strains but also through the shifts in the abundance of several cytochrome P450 (CYP450s). Overall, resistance to deltamethrin was linked in the isofemale strains to resistance to other class of insecticides, suggesting that cross- and multiple resistance occur through selection of mechanisms of metabolic resistance. These results give some insights into resistance to deltamethrin and into multiple resistance phenomena in populations of Ae. aegypti. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/33428654; PUBMED: 33428654; PUBMEDCENTRAL: PMC7799788 |
| DOI: |
10.1371/journal.pone.0243992 |
| Availability: |
https://pasteur.hal.science/pasteur-03184205; https://pasteur.hal.science/pasteur-03184205v1/document; https://pasteur.hal.science/pasteur-03184205v1/file/journal.pone.0243992.pdf; https://doi.org/10.1371/journal.pone.0243992 |
| Rights: |
http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.457DFDD9 |
| Database: |
BASE |