| Title: |
Vascular O2.‐ and H2O2 production and oxidative stress resistance in two closely related rodent species with disparate longevity |
| Authors: |
Csiszar, Anna; Labinskyy, Nazar; Ballabh, Praveen; Austad, Steven N; Podlutsky, Andrej; Ungvari, Zoltan |
| Source: |
The FASEB Journal ; volume 22, issue S1 ; ISSN 0892-6638 1530-6860 |
| Publisher Information: |
Wiley |
| Publication Year: |
2008 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Vascular aging is characterized by increased oxidative stress, impaired NO bioavailability and enhanced apoptotic cell death. The oxidative stress hypothesis of aging predicts that vascular cells of long‐lived species exhibit lower production of ROS and/or superior resistance to oxidative stress. We tested this hypothesis using two taxonomically related rodents, the white‐footed mouse (Peromyscus leucopus) and M. musculus (lifespan: 8 vs. 3.5 years). Compared to mice, in aortas of P. leucopus there were 1) a lower NADPH oxidase expression/activity, endothelial O2.‐/H2O2 production and ROS generation by mitochondria, 2) increased expression of catalase, Gpx‐1, HO‐1 and eNOS, and 3) increased NO production. In mouse aortas treatment with ox‐LDL elicited oxidative stress, endothelial dysfunction and apoptosis, whereas vessels of P. leucopus were resistant to deleterious effects of oxLDL and H2O2. Primary fibroblasts from P. leucopus also exhibited less H2O2 ‐induced DNA damage. Thus, increased lifespan potential in P. leucopus is associated with a decreased cellular ROS generation and increased oxidative stress resistance, which accord with the prediction of the oxidative stress hypothesis of aging. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1096/fasebj.22.1_supplement.747.3 |
| Availability: |
http://dx.doi.org/10.1096/fasebj.22.1_supplement.747.3 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.45CEC68B |
| Database: |
BASE |