| Title: |
Effect Of combining FK506 and neurotrophins on neurite branching and elongation |
| Authors: |
Labroo, Pratima; Shea, Jill; Sant, Himanshu; Gale, Bruce; Agarwal, Jayant |
| Contributors: |
U.S. Department of Defense |
| Source: |
Muscle & Nerve ; volume 55, issue 4, page 570-581 ; ISSN 0148-639X 1097-4598 |
| Publisher Information: |
Wiley |
| Publication Year: |
2016 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Introduction There is a clinical need to improve the outcomes of peripheral nerve regeneration and repair after injury. In addition to its immunosuppressive effects, FK506 (tacrolimus) has been shown to have neuroregenerative properties. To determine biologically relevant local FK506 and growth factor concentrations, we performed an in vitro bioassay using dorsal root ganglion (DRG) from chicken embryos. Methods Neurite elongation and neurite branching were analyzed microscopically after addition of FK506, glial cell line‐derived neurotrophic factor (GDNF), and nerve growth factor (NGF), each alone and in combination. Results FK506 induced modest neurite elongation (∼500–800 µm) without improving neurite branching significantly. The combination of FK506 with NGF, GDNF, or both, exerted a potentiating or competitive effect on neurite elongation (∼700–1100 µm) based on dosage and competitive effect on neurite branching (∼0.2–0.4). Conclusions These results strongly suggest that the interaction of FK506 with GDNF and NGF mediates distinct enhancement of neurite growth. Muscle Nerve 55 : 570–581, 2017 |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1002/mus.25370 |
| Availability: |
https://doi.org/10.1002/mus.25370; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmus.25370; https://onlinelibrary.wiley.com/doi/pdf/10.1002/mus.25370; https://onlinelibrary.wiley.com/doi/full-xml/10.1002/mus.25370; http://api.wiley.com/onlinelibrary/chorus/v1/articles/10.1002%2Fmus.25370 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.461AD256 |
| Database: |
BASE |