| Title: |
Protocol for microbial profiling of low-biomass upper respiratory tract samples |
| Authors: |
Odendaal,Mari-Lee; Chu, Mei Ling J N; Arp,Kayleigh; van Beveren, Gina J; Biesbroek,Giske; Binkowska,Justyna; Bosch,Thijs; Groot,James A; Hasrat, Raiza; Kuiling,Sjoerd; van Logchem,Elske M; Parga,Paula Lusarreta; de Steenhuijsen Piters, Wouter A A; Bogaert, Debby; MMB-res; MMB-mcb; Infection & Immunity; Infectieziekten onderzoek3 (Bogaert); Child Health; Onderzoek |
| Publication Year: |
2025 |
| Subject Terms: |
Journal Article |
| Description: |
The upper respiratory tract (URT) microbiota plays a role in both acute and chronic respiratory health outcomes and consists of multiple ecologically distinct niches, all of which have low bacterial biomass. Here, we present a protocol for microbial profiling of low-biomass URT samples. We describe steps for collecting, storing, and extracting DNA. We then detail procedures for performing 16S rRNA sequencing, using an Illumina MiSeq platform, to characterize microbial communities. For complete details on the use and execution of this protocol, please refer to Odendaal et al.1. |
| Document Type: |
article in journal/newspaper |
| File Description: |
text/plain |
| Language: |
English |
| ISSN: |
2666-1667 |
| Relation: |
https://dspace.library.uu.nl/handle/1874/461093 |
| Availability: |
https://dspace.library.uu.nl/handle/1874/461093 |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.467E1454 |
| Database: |
BASE |