| Title: |
Could PLATELIA Toxo IgM be the new gold standard for the serological diagnosis of congenital toxoplasmosis: a French multicenter study |
| Authors: |
Arkhis, Safya; Rouges, Célia; Dahane, Naima; Guegan, Hélène; Yera, Hélène; Robert-Gangneux, Florence |
| Contributors: |
Centre Hospitalier Universitaire de Rennes CHU Rennes = Rennes University Hospital Pontchaillou; Hôpital Cochin AP-HP; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP); École des Hautes Études en Santé Publique EHESP (EHESP); Institut de recherche en santé, environnement et travail (Irset); Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique EHESP (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes (Biosit : Biologie - Santé - Innovation Technologique); Hôpital Dupuytren CHU Limoges |
| Source: |
ISSN: 0095-1137 ; Journal of Clinical Microbiology ; https://hal.science/hal-04444019 ; Journal of Clinical Microbiology, 2024, Journal of Clinical Microbiology, 62 (2), pp.e0122223. ⟨10.1128/jcm.01222-23⟩. |
| Publisher Information: |
HAL CCSD; American Society for Microbiology |
| Publication Year: |
2024 |
| Collection: |
Université de Rennes 1: Publications scientifiques (HAL) |
| Subject Terms: |
IgM; Toxoplasma gondii; Congenital toxoplasmosis; Neonatal diagnosis; Serology; [SDV]Life Sciences [q-bio] |
| Description: |
International audience ; To assess the performance of PLATELIA Toxo IgM (Bio-Rad) and Toxo ISAGA (BioMérieux) to detect anti- IgM in infants at risk of congenital toxoplasmosis, a retrospective multicenter study was conducted comparing serological results obtained in the framework of routine diagnosis work-up for congenital toxoplasmosis. All infants born to mothers infected with during pregnancy from 2010 to 2020 with at least 6 months of serological follow-up were included ( = 1,010). One thousand ten cases were included, of which 250 infants (24.75%) had congenital toxoplasmosis. A total of 1039 sera were included. The concordance between the two techniques was 96%, with kappa coefficient of 0.87, showing an almost perfect agreement between ISAGA and PLATELIA. Cumulative sensitivity and specificity were 73.2% and 99.5.% and 74.8% and 100% for ISAGA and PLATELIA, respectively. The mean time to detect IgM using ISAGA and PLATELIA tests was 6.9 ± 20.1 days and 5.6 ± 14.7 days, respectively not significant (ns). Finally, the sensitivity of ISAGA and PLATELIA to detect IgM antibodies in infected neonates at 5 days of life was 62% and 64%, respectively. Performances of PLATELIA Toxo IgM assay were comparable to the gold standard ISAGA. This enzyme-linked immunosorbent assay is suitable for routine serology for the diagnosis of congenital toxoplasmosis in newborns. IMPORTANCE This study will help clinical microbiologists to chose an alternative serological method for the neonatal diagnosis of congenital toxoplasmosis, once the gold standard technique ISAGA will be withdrawn next year. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/38259072; PUBMED: 38259072 |
| DOI: |
10.1128/jcm.01222-23 |
| Availability: |
https://hal.science/hal-04444019; https://hal.science/hal-04444019v1/document; https://hal.science/hal-04444019v1/file/Arkhis%20et%20al%202024-Could%20PLATELIA%20Toxo%20IgM%20be%20the%20new%20gold%20standard%20for%20the%20serological%20diagnosis%20of%20congenital%20toxoplasmosis%20a%20French%20multicenter%20study.pdf; https://doi.org/10.1128/jcm.01222-23 |
| Rights: |
http://creativecommons.org/licenses/by-nc/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.46BDD20A |
| Database: |
BASE |