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Co-occurring microbial guilds in pig fecal microbiota: key drivers and effects on host performance

Title: Co-occurring microbial guilds in pig fecal microbiota: key drivers and effects on host performance
Authors: Vourlaki, Ioanna-Theoni; Río-López, Raquel; Clavell Sansalvador, Adrià; Ramírez Ayala, Lino César; Ballester Devis, Maria; Sanchez, Juan Pablo; Piles, Miriam; Quintanilla, Raquel; da Fonseca de Oliveira, Angela C.; Batista Costa, Leandro; DALMAU, ANTONI; Ramayo-Caldas, Yuliaxis
Contributors: Producció Animal; Benestar Animal; Genètica i Millora Animal
Publisher Information: BioMed Central
Publication Year: 2025
Collection: IRTA Pubpro (Institute of Agrifood Research and Technology / Institut de Recerca i Tecnologia Agroalimentàries)
Time: 636
Description: Background The pig gut microbiota is a complex ecosystem composed of microbial guilds that remain largely unexplored. Here we decomposed the pig fecal microbiota of two cohorts of 648 healthy Duroc pigs during the transition (n = 400) and growing finish (n = 248) periods in co-occurring bacterial guilds defined as pig enterosignatures (ES). Results Our results indicate that fecal microbial ecosystems can accurately be described by combinations of at least six ES, driven by the Prevotella (ES-Prev), Treponema (ES-Trep), Lactobacillus (ES-Lact), Clostridium (ES-Clost), Streptococcus (ES-Strep), and UBA2810 (ES-UBA2) genera. We observed a dynamic shift with age in the composition of ES, where ES-Prev, ES-Strep, and ES-Lact seem to be core components. Our results suggest partial genetic control by the host, with heritabilities of ES composition ranging from 0.24 to 0.36. Furthermore, our findings indicate that stress on the host is associated with assembly of the ES, decreasing ES-Lact abundance, and increasing prevalence of ES-Strep. We noted a positive association of ES-Prev with growth rate at 60-days, which later evolved to become negative, impacting feed efficiency during the growing period. Remarkably, a negative association of the abundance of ES-Lact with levels of hair cortisol was also found during this period. Conclusions Our findings provide novel insights into the pig gut microbiota and reveal novels associations with relevant porcine physiological and performance traits. Moreover, while the ES concept has proven valuable in dissecting microbial communities into assemblies of underlying microbial guilds, our results emphasize the relevance of customizing microbial interventions strategies based on the nutritional and health requirements at each stage of the porcine production cycle. ; ITV was funded by the HoloRuminant (101000213) and GEroNIMO (101000236) H2020 projetcs. AC was funded with a PhD fellowship (PRE2022-101829) awarded by the Spanish Ministry of Education and Culture. YRC received the “Ramon ...
Document Type: article in journal/newspaper
File Description: application/pdf
Language: English
Relation: Genetics Selection Evolution; EC/H2020/101000213/EU/Understanding microbiomes of the ruminant holobiont/HoloRuminant; EC/H2020/101000236/EU/Genome and Epigenome eNabled breedIng in MOnogastrics/GEroNIMO; MICIU/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC2019-027244-I/ES/Metagenomics and integrative biology tools to improve sustainable livestock systems/; MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2017-88849-R/ES/MICROBIOTA INTESTINAL Y GENETICA DEL HUESPED: CONTRIBUCION CONJUNTA A LA EFICIENCIA, EL COMPORTAMIENTO Y LA ROBUSTEZ EN PORCINO/; MICINN/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112677RB-C21/ES/FISIOLOGIA MOLECULAR DEL INMUNOMETABOLISMO EN PORCINO: BASES PARA LA SELECCION DE POBLACIONES MAS ROBUSTAS/; MICINN/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/PID2021-126555OB-I00/ES/ESTUDIO DE LAS INTERELACIONES ENTRE LA MICROBIOTA DEL INTESTINO Y EL COMPORTAMIENTO ANIMAL (EJE INTESTINO-CEREBRO) EN PORCINO DE ENGORDE/; https://hdl.handle.net/20.500.12327/4588
DOI: 10.1186/s12711-025-00979-x
Availability: https://hdl.handle.net/20.500.12327/4588; https://doi.org/10.1186/s12711-025-00979-x
Rights: Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/openAccess
Accession Number: edsbas.47C5A855
Database: BASE