| Title: |
Interleukin-1β induces trained innate immunity in human hematopoietic progenitor cells in vitro |
| Authors: |
Flores-Gomez, D; Hobo, W; van Ens, D; Kessler, EL; Novakovic, B; Schaap, N; Rijnen, WHC; Joosten, LAB; Netea, MG; Riksen, NP; Bekkering, S |
| Publisher Information: |
Elsevier BV |
| Publication Year: |
2024 |
| Collection: |
The University of Melbourne: Digital Repository |
| Description: |
Innate immune cells can develop a long-lasting hyperresponsive phenotype, termed trained immunity, mediated by epigenetic and metabolic reprogramming. In mice, exposure to Bacille Calmette-Guérin (BCG), β-glucan, or Western diet induces trained immunity by reprogramming hematopoietic progenitor cells (HPCs), through interleukin-1β (IL-1β) signaling in the bone marrow (BM). We investigated whether IL-1β induces trained immunity in primary human BM-derived HPCs in vitro. We exposed human BM-derived HPCs to IL-1β for 4 h. HPCs were expanded and differentiated into monocytes followed by functional and transcriptomic characterization. IL-1β-exposed HPCs showed higher granulocyte-macrophage colony-forming units. The monocyte offspring produced more tumor necrosis factor (TNF) and IL-1β after restimulation with lipopolysaccharide (LPS) and Pam3Cys and is metabolically more active. Transcriptomic analysis showed upregulation of key atherogenic and inflammatory pathways. In conclusion, brief exposure of human BM-derived HPCs to IL-1β in vitro induces a trained immunity phenotype. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| ISSN: |
2213-6711 |
| Relation: |
https://hdl.handle.net/11343/359235 |
| Availability: |
https://hdl.handle.net/11343/359235 |
| Rights: |
https://creativecommons.org/licenses/by/4.0 ; CC BY |
| Accession Number: |
edsbas.48176734 |
| Database: |
BASE |