| Title: |
Ivermectin and dexamethasone combination induces apoptosis in SUP-B15 cell line |
| Authors: |
Siregar, Olga R.; Wahyuni, Arlinda S.; Pasaribu, Ayodhia P.; Edianto, Deri; Ugrasena, I DG.; Amelia, Rina; Lubis, Inke ND.; Rusda, Muhammad |
| Source: |
Narra J; Vol. 5 No. 2 (2025): August 2025; e1975 ; 2807-2618 |
| Publisher Information: |
Narra Sains Indonesia |
| Publication Year: |
2025 |
| Subject Terms: |
Acute lymphoblastic leukemia; apoptosis; dexamethasone; glucocorticoid resistance; ivermectin |
| Description: |
The development of glucocorticoid resistance has complicated the management of acute lymphoblastic leukemia (ALL), leading to increased mortality rates. Ivermectin, a low-cost and well-established anthelmintic, exhibits anticancer potential and may enhance glucocorticoid toxicity in ALL, offering a possible strategy to overcome resistance. The aim of this study was to evaluate the apoptotic effect of combining ivermectin with dexamethasone in ALL. ALL SUP-B15 cells were cultured under standard conditions before treatment with dexamethasone (200 nM) alone or combined with ivermectin (5, 10, and 20 µM), with an untreated group serving as the control. Cytotoxicity was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay by measuring cell viability and inhibition. Apoptosis was evaluated through BAX, BCL-2, and CASP3 gene expression analysis using reverse transcription-polymerase chain reaction (RT-PCR). The findings revealed that the combination of ivermectin and dexamethasone was superior in the repression of ALL cell viability compared to control (p |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
https://narraj.org/main/article/view/1975/846; https://narraj.org/main/article/view/1975 |
| DOI: |
10.52225/narra.v5i2.1975 |
| Availability: |
https://narraj.org/main/article/view/1975; https://doi.org/10.52225/narra.v5i2.1975 |
| Rights: |
Copyright (c) 2025 Olga R. Siregar, Arlinda S. Wahyuni, Ayodhia P. Pasaribu, Deri Edianto, I DG. Ugrasena, Rina Amelia, Inke ND. Lubis, Muhammad Rusda ; https://creativecommons.org/licenses/by-nc/4.0 |
| Accession Number: |
edsbas.48355A3F |
| Database: |
BASE |