| Title: |
Moving into the red – a near infra-red optical probe for analysis of human neutrophil elastase in activated neutrophils and neutrophil extracellular traps |
| Authors: |
Rodriguez Rios, Maria; Megía Fernández, Alicia |
| Publisher Information: |
Royal Society of Chemistry |
| Publication Year: |
2023 |
| Collection: |
DIGIBUG: Repositorio Institucional de la Universidad de Granada |
| Description: |
Neutrophils are the first immune cells recruited for defence against invading pathogens; however, their dysregulated activation and subsequent release of the enzyme human neutrophil elastase is associated with several, inflammation-based, diseases. Herein, we describe a FRET-based, tri-branched (one quencher, three fluorophores) near infrared probe that provides an intense OFF/ON amplified fluorescence signal for specific detection of human neutrophil elastase. The probe allowed selective detection of activated neutrophils and labelling of neutrophil extracellular traps. A tri-branched, FRET-based probe for the detection of hNE was synthesised with emission in the NIR region of the spectrum, where endogenous biomolecules have decreased absorbance, and minimal autofluorescence ; UK Research & Innovation (UKRI) Engineering & Physical Sciences Research Council (EPSRC) EP/R005257/1 ; UK Research & Innovation (UKRI) Medical Research Council UK (MRC) MR/N02995X/1 |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
Chem. Commun., 2023, 59, 11660[DOI:10.1039/d3cc03634k]; https://hdl.handle.net/10481/85243 |
| DOI: |
10.1039/d3cc03634k |
| Availability: |
https://hdl.handle.net/10481/85243; https://doi.org/10.1039/d3cc03634k |
| Rights: |
Atribución 4.0 Internacional ; http://creativecommons.org/licenses/by/4.0/ ; open access |
| Accession Number: |
edsbas.48C3C07 |
| Database: |
BASE |