| Title: |
Ιn vitro μελέτη της ικανότητας διαφοροποίησης των μεγάλων μονοπυρήνων του περιφερικού αίματος προς κύτταρα που παράγουν ινσουλίνη ; In vitro study of the differentiation ability of peripheral blood monocytes into insulin-producing cells |
| Authors: |
Kyventidis, Anastasios; Κυβεντίδης, Αναστάσιος |
| Publisher Information: |
Aristotle University Of Thessaloniki (AUTH); Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ) |
| Publication Year: |
2016 |
| Collection: |
National Archive of PhD Theses (National Documentation Centre Greece) |
| Subject Terms: |
διαφοροποίηση μονοκυττάρων; ινσουλινο-παραγωγά κύτταρα; Σακχαρώδης διαβήτης; Λιραγλουτίδη; monocyte differentiation; insulin-producing cells; Diabetes mellitus; liraglutide; Ιατρική και Επιστήμες Υγείας; Βασική Ιατρική; Medical and Health Sciences; Basic Medicine |
| Description: |
Introduction. Recent studies provide evidence that peripheral blood monocytes have the ability to differentiate to mesenchymal-like cells. The aim of the present study is to analyse the ability of cultured monocytes to de-differentiate and produce insulin in vitro using growth factors or not and a long acting analog of Glucagon-Like-Peptide-1. The effect of a long acting analog of Glucagon-Like-Peptide-1 to monocyte differentiation was studied for the first time. (2)Methods Peripheral blood monocytes were isolated from healthy donors and cultivated for fourteen days. Growth factors and liraglutide were used to induce pancreatic differentiation in most of the cultures. The growth factors were: monocyte colony-stimulating factor, interleukin-3, hepatocyte growth factor and epidermal growth factor. The rest of the cultures were cultivated only with nutrient medium RPMI and human serum. Insulin levels were measured with an enzyme-linked immunosorbent assay. Measurements took place at the beginning of the cultures, on the seventh day and on the fourteenth day. Cellular morphology was observed using optical and electron microscopy. Cells were stained with May Grunwald-Giemsa dye. Cell membrane receptors were detected by flow cytometry. Statistical analysis was performed using parametric and non parametric statistical tests, depending on the normality of the distribution of the samples. (3)Results Monocytes were able to synthesize and excrete high levels of insulin after seven days in culture. Α further increase in the excretion of insulin was observed after fourteen days. Cells were also able to de-differentiate and synthesize insulin, even if no growth factors and liraglutide were added in the culture medium. The production of insulin is not statistically different between the cultures cultivated in the presence of growth factors and liraglutide and the cultures cultivated in the absence of growth factors. Differentiated monocytes were connected to neighbouring cells with axons. The morphology of the whole resembled ... |
| Document Type: |
doctoral or postdoctoral thesis |
| Language: |
Greek, Modern (1453-) |
| Relation: |
https://hdl.handle.net/10442/hedi/38189 |
| DOI: |
10.12681/eadd/38189 |
| Availability: |
https://hdl.handle.net/10442/hedi/38189; https://doi.org/10.12681/eadd/38189 |
| Accession Number: |
edsbas.4A05B0E2 |
| Database: |
BASE |