| Title: |
Metalloproteinase-9 gene variants and risk for hypertension among ethnic Javanese |
| Authors: |
Fitranto Arjadi; Saefuddin ‘Aziz; Alfi Muntafiah |
| Source: |
Universa Medicina, Vol 33, Iss 2, Pp 213-220 (2015) |
| Publisher Information: |
Faculty of Medicine Trisakti University |
| Publication Year: |
2015 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
Hypertension; ethnic Javanese; MMP-9 gene polymorphism; adults; Medicine |
| Description: |
Background Hypertension is associated with endothelial-dependent vasodilation disorders, due to reduced nitric oxide (NO) availability and excessive angiotensin II (ANG-II) activation. The objective of this study was to determine the association between matrix metallopeptidase 9 (MMP-9) gene polymorphism and hypertension in ethnic Javanese in the 40-80 year age group. Methods This was a case-control study on 50 PROLANIS patients of family doctors meeting the inclusion criteria and 50 controls without hypertension. Subjects were hypertensive patients with constant systolic arterial pressure of >140 mmHg and diastolic arterial pressure of >90 mmHg, confirmed in three successive measurements The observed parameters were degree of MMP-9 polymorphism, and NO and ANG-II levels. Matrix metallopeptidase 9 polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using the SmaI restriction enzyme. MMP-9 polymorphisms were indicated by variation in band patterns. Degree of polymorphism in cases and controls were compared with NO and ANG-II levels in both groups. Data analysis was done using independent t-test. Results The heterozygous (3 band) to normal (2 band) MMP-9 genotype ratio was 3:1 in hypertensives, but balanced in controls. In hypertensives, heterozygous GA and homozygous AA genotype frequencies were respectively 3.198 and 1.548 times higher than that of the GG genotype (p=0.008 and p=0.726). There was a statistically significant differences of NO and Ang-II levels between cases and controls (p=0.000 and p=0.000; respectively). Conclusion Matrix metallopeptidase 9 gene polymorphisms in hypertensive ethnic Javanese are associated with NO and angiotensin II levels. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
http://www.univmed.org/wp-content/uploads/2015/03/Fitranto.pdf; https://doaj.org/toc/1907-3062; https://doaj.org/article/0af06e7e6cad44f08d727d9ca40381d7 |
| Availability: |
https://doaj.org/article/0af06e7e6cad44f08d727d9ca40381d7 |
| Accession Number: |
edsbas.4A982A8A |
| Database: |
BASE |