| Title: |
Administration of DNA Encoding the Interleukin‐27 Gene Augments Antitumour Responses through Non‐adaptive Immunity |
| Authors: |
Li, Q.; Sato, A.; Shimozato, O.; Shingyoji, M.; Tada, Y.; Tatsumi, K.; Shimada, H.; Hiroshima, K.; Tagawa, M. |
| Contributors: |
Ministry of Education, Culture, Sports, Science and Technology of Japan; Ministry of Health, Labor and Welfare of Japan; Nichias Corporation |
| Source: |
Scandinavian Journal of Immunology ; volume 82, issue 4, page 320-327 ; ISSN 0300-9475 1365-3083 |
| Publisher Information: |
Wiley |
| Publication Year: |
2015 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
DNA ‐mediated immunization of a tumour antigen is a possible immunotherapy for cancer, and interleukin ( IL )‐27 has diverse functions in adaptive immunity. In this study, we examined whether IL ‐27 DNA administration enhanced antitumour effects in mice vaccinated with DNA encoding a putative tumour antigen, β ‐galactosidase ( β ‐gal). An intramuscular injection of cardiotoxin before DNA administration facilitated the exogenous gene expression. In mice received β ‐gal and IL ‐27 DNA , growth of β ‐gal‐positive P815 tumours was retarded and survival of the mice was prolonged. Development of β ‐gal‐positive Colon 26 tumours was suppressed by vaccination of β ‐gal DNA and further inhibited by additional IL ‐27 DNA administration or IL ‐12 family cytokines. Nevertheless, a population of β ‐gal‐specific CD 8 + T cells did not increase, and production of anti‐ β ‐gal antibody was not enhanced by IL ‐27 DNA administration. Spleen cells from mice bearing IL ‐27‐expressing Colon 26 tumours showed greater YAC ‐1‐targeted cytotoxicity although CD 3 − / DX 5 + natural killer ( NK ) cell numbers remained unchanged. Recombinant IL ‐27 enhanced YAC ‐1‐targeted cytotoxicity of IL ‐2‐primed splenic NK cells and augmented a phosphorylation of signal transducer and activator of transcription 3 and an expression of perforin. These data collectively indicate that IL ‐27 DNA administration activates NK cells and augments vaccination effects of DNA encoding a tumour antigen through non‐adaptive immune responses. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1111/sji.12321 |
| Availability: |
https://doi.org/10.1111/sji.12321; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fsji.12321; https://onlinelibrary.wiley.com/doi/pdf/10.1111/sji.12321 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.4BFBDEE3 |
| Database: |
BASE |