| Title: |
Additional single cell RNA-seq data. |
| Authors: |
Jennifer VanOudenhove; Yuxin Liu; Raman Nelakanti; Dongjoo Kim; Emma Busarello; Natalia Tijaro Ovalle; Zhihong Qi; Padmavathi Mamillapalli; Alexa Siddon; Zhiliang Bai; Alfredo Axtmayer; Cheryl Corso; Shalin Kothari; Francine Foss; Iris Isufi; Toma Tebaldi; Lohith Gowda; Rong Fan; Stuart Seropian; Stephanie Halene |
| Publication Year: |
2025 |
| Subject Terms: |
Microbiology; Cell Biology; Genetics; Molecular Biology; Neuroscience; Immunology; Developmental Biology; Cancer; Mental Health; Infectious Diseases; Virology; transplant remains unclear; studies revealed defects; preventing severe disease; elevated mortality rates; 5 healthy controls; 10 &# 8211; 2 vaccine response; 2 mrna vaccines; adaptive immune responses; 42 hsct recipients; 2 infection; immune profiling; vaccination responses; cellular responses; %22">xlink ">; spike igg; selected based; nucleocapsid levels; myeloablative chemotherapy |
| Description: |
A. Table of characteristics of the individuals profiled by single cell RNA-seq. B. Distribution of Feature Counts per individual broken out by vaccination time point. C. Mitochondrial Read Percentage per individual broken out by vaccination time point. D. Heatmap showing the top three marker genes for each T cell cluster in Fig 4B . E. Cells from the UMAP projection in Fig 4D were split by individual and colored by cluster annotation. F. Gene set enrichment analysis with Enrichr of the differential gene expression between good and poor T cell responders post vaccination (left). Gene Expression of Central Memory T cell markers that are at the top of the differential gene expression table when comparing the gene expression post vaccination of the good versus poor T cell responders (right). These genes are SELL/CD62L , CCR7 , TCF7 , and CD27 . G. Data was filtered to include only B cell clusters and excluded two post-COVID-19 infection samples. UMAP projection of the B cells re-clustered and re-annotated as with T cells. H. Heatmap showing the top marker genes for each B cell cluster in S2 Fig 2G I. Unique B cell clonotype number for each individual at each time point. (PDF) |
| Document Type: |
article in journal/newspaper |
| Language: |
unknown |
| DOI: |
10.1371/journal.pone.0320744.s002 |
| Availability: |
https://doi.org/10.1371/journal.pone.0320744.s002; https://figshare.com/articles/journal_contribution/Additional_single_cell_RNA-seq_data_/28885264 |
| Rights: |
CC BY 4.0 |
| Accession Number: |
edsbas.4D2DC71F |
| Database: |
BASE |