| Title: |
3D single-molecule super-resolution imaging of microfabricated multiscale fractal substrates for calibration and cell imaging |
| Authors: |
Cabriel, Clément; Córdova-Castro, R. Margoth; Berenschot, Erwin; Dávila-Lezama, Amanda; Pondman, Kirsten; Le Gac, Séverine; Tas, Niels; Susarrey-Arce, Arturo; Izeddin, Ignacio |
| Contributors: |
Institut Langevin - Ondes et Images (UMR7587) (IL); Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris); Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS); University of Ottawa Ottawa; University of Twente; Universidad Autónoma de Baja California (UABC); ANR-20-CE45-0023,ABC4M,Microscopie multimodale assistée par calcul Bayésien approximé pour explorer la mobilité nucléaire des facteurs de transcription(2020); ANR-20-CE24-0021,SP-Tunnel-OHG,Génération d'harmoniques d'ordre élevé dans les jonctions tunnel plasmoniques(2020) |
| Source: |
ISSN: 1944-8244. |
| Publisher Information: |
CCSD; American Chemical Society |
| Publication Year: |
2025 |
| Subject Terms: |
calibration; material imaging; fractallike microstructures; multiscale material; bioimaging; 3D single-molecule localization microscopy; [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry; Molecular Biology/Biophysics; [CHIM.MATE]Chemical Sciences/Material chemistry; [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics; [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic |
| Description: |
International audience ; Microstructures arrayed over a substrate have shown increasing interest due to their ability to provide advanced 3D cellular models, which open new possibilities for cell culture, proliferation, and differentiation. Still, the mechanisms by which physical cues impact the cell phenotype are not fully understood, hence the necessity to interrogate cell behavior at the highest resolution. However, cell 3D high-resolution optical imaging on such microstructured substrates remains challenging due to their complexity, as well as axial calibration issues. In this work, we address this issue by leveraging the geometrical characteristics of fractal-like structures, which serve as axial calibration tools and to modulate cell growth. To this end, we use multiscale 3D SiO 2 substrates consisting of spatially arrayed octahedral features of a few micrometers to hundreds of nanometers. Through optimizations of both the structures and optical imaging conditions, we demonstrate the potential of these 3D multiscale structures as an alternative to electron microscopy for material imaging, but also as calibration tools for 3D super-resolution microscopy. We use their multiscale and known geometry to perform lateral and axial calibrations in 3D single-molecule localization microscopy (SMLM) and assess imaging resolutions. We then utilize these substrates as a platform for high-resolution bioimaging. As proof of concept, we cultivate human mesenchymal stem cells on these substrates, revealing very different growth patterns compared to flat glass. Specifically, the spatial distribution of cytoskeleton proteins is vastly modified, as we demonstrate with 3D SMLM assessment. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1021/acsami.4c19431 |
| Availability: |
https://hal.science/hal-05568786; https://hal.science/hal-05568786v1/document; https://hal.science/hal-05568786v1/file/Manuscript_fractals_main.pdf; https://doi.org/10.1021/acsami.4c19431 |
| Rights: |
https://creativecommons.org/licenses/by-nc/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.4D7E229E |
| Database: |
BASE |