| Title: |
Temperature-dependent elastic properties of DNA |
| Authors: |
Marc Rico-Pasto; Felix Ritort |
| Source: |
Biophysical Reports, Vol 2, Iss 3, Pp 100067- (2022) |
| Publisher Information: |
Elsevier |
| Publication Year: |
2022 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
Physics; QC1-999; Biology (General); QH301-705.5 |
| Description: |
Knowledge of the elastic properties, e.g., the persistence length or interphosphate distance, of single-stranded (ss) and double-stranded (ds) DNA under different experimental conditions is critical to characterizing molecular reactions studied with single-molecule techniques. While previous experiments have addressed the dependence of the elastic parameters upon varying ionic strength and contour length, temperature-dependent effects are less studied. Here, we examine the temperature-dependent elasticity of ssDNA and dsDNA in the range 5°C–50°C using a temperature-jump optical trap. We find a temperature softening for dsDNA and a temperature stiffening for ssDNA. Our results highlight the need for a general theory explaining the phenomenology observed. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2667074722000246; https://doaj.org/toc/2667-0747; https://doaj.org/article/67e54e28ed4e4de69e649fe02b417f08 |
| DOI: |
10.1016/j.bpr.2022.100067 |
| Availability: |
https://doi.org/10.1016/j.bpr.2022.100067; https://doaj.org/article/67e54e28ed4e4de69e649fe02b417f08 |
| Accession Number: |
edsbas.4E18DE50 |
| Database: |
BASE |