| Title: |
Dynein arms are strain‐dependent direction‐switching force generators |
| Authors: |
Shingyoji, Chikako; Nakano, Izumi; Inoue, Yuichi; Higuchi, Hideo |
| Contributors: |
Japan Society for the Promotion of Science |
| Source: |
Cytoskeleton ; volume 72, issue 8, page 388-401 ; ISSN 1949-3584 1949-3592 |
| Publisher Information: |
Wiley |
| Publication Year: |
2015 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Dynein is a minus‐end‐directed motor that can generate (forward) force to move along the microtubule toward its minus end. In addition, axonemal dyneins were reported to oscillate in the generation of forward force, and cytoplasmic dynein is observed to generate bidirectional forces in response to defined chemical states. Both dyneins can also respond to mechanically applied force. To test whether axonemal dynein can switch direction of force generation, we measured force using an optical trap and UV‐photolysis of caged ATP. We observed that isolated dynein could repeatedly generate force in both directions along the microtubule. Bidirectional force was also observed for dynein arms that are still attached on the doublet microtubules. Axonemal dynein generated force to move backward (∼4 pN) as well as forward (5‐6 pN) along microtubules. Furthermore, backward force could be stimulated by plus‐end directed external force applied to axonemal dynein before ATP application. The results show that axonemal dynein is unique exhibiting multiple modes of force generation including backward and forward force, oscillatory force and slow, repetitive bidirectional force. The results also demonstrate that mechanical strain is important for switching the directionality of force generation in axonemal dyneins. © 2015 Wiley Periodicals, Inc. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1002/cm.21232 |
| Availability: |
https://doi.org/10.1002/cm.21232; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fcm.21232; https://onlinelibrary.wiley.com/doi/pdf/10.1002/cm.21232 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.4F2DB36C |
| Database: |
BASE |