| Title: |
Circular RNAs regulate neuron size and migration of midbrain dopamine neurons during development |
| Authors: |
Rybiczka-Tešulov, Mateja; Garritsen, Oxana; Venø,Morten T.; Wieg, Laura; Dijk, Roland van; Rahimi,Karim; Gomes-Duarte, Andreia; Wit, Marina de; van de Haar, Lieke L.; Michels,Lars; van Kronenburg, Nicky C.H.; van der Meer,Christiaan; Kjems,Jørgen; Vangoor, Vamshidhar R.; Pasterkamp, R. Jeroen; TN groep Pasterkamp; Brain; TN groep Hol; Onderzoek NCH; Translational Neuroscience; Regenerative Medicine and Stem Cells |
| Publication Year: |
2024 |
| Subject Terms: |
General Chemistry; General Biochemistry,Genetics and Molecular Biology; General Physics and Astronomy |
| Description: |
Midbrain dopamine (mDA) neurons play an essential role in cognitive and motor behaviours and are linked to different brain disorders. However, the molecular mechanisms underlying their development, and in particular the role of non-coding RNAs (ncRNAs), remain incompletely understood. Here, we establish the transcriptomic landscape and alternative splicing patterns of circular RNAs (circRNAs) at key developmental timepoints in mouse mDA neurons in vivo using fluorescence-activated cell sorting followed by short- and long-read RNA sequencing. In situ hybridisation shows expression of several circRNAs during early mDA neuron development and post-transcriptional silencing unveils roles for different circRNAs in regulating mDA neuron morphology. Finally, in utero electroporation and time-lapse imaging implicate circRmst, a circRNA with widespread morphological effects, in the migration of developing mDA neurons in vivo. Together, these data for the first time suggest a functional role for circRNAs in developing mDA neurons and characterise poorly defined aspects of mDA neuron development. |
| Document Type: |
article in journal/newspaper |
| File Description: |
text/plain |
| Language: |
English |
| ISSN: |
2041-1723 |
| Relation: |
https://dspace.library.uu.nl/handle/1874/455242 |
| Availability: |
https://dspace.library.uu.nl/handle/1874/455242 |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.4F916366 |
| Database: |
BASE |