| Title: |
Standard units for ElectroChromic Shift measurements in plant biology |
| Authors: |
Mathiot, Charlie; Alric, Jean |
| Contributors: |
Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM); Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA); CEA Tech en régions (CEA-TECH-Reg); Direction de Recherche Technologique (CEA) (DRT (CEA)); Photosynthèse & Environnement (P&E); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)); This work was supported by CEA, CNRS and grant from Region Sud APR-EX 2019-03086 and 2016-03216 |
| Source: |
ISSN: 0022-0957. |
| Publisher Information: |
CCSD; Oxford University Press (OUP) |
| Publication Year: |
2021 |
| Collection: |
Aix-Marseille Université: HAL |
| Subject Terms: |
ATP-synthase; cyclic electron flow; thylakoid; chloroplast; Photosynthesis; [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics |
| Description: |
International audience ; The absorbance shift of pigments is proportional to the membrane potential (Δψ) in plants, green algae, and many photosynthetic bacteria. It is currently denoted as ElectroChromic Shift (ECS) at 515–520 nm for plant carotenoids. It is increasingly being used for phenotyping plants for traits related to photosynthesis or chloroplast metabolism because it is a non-invasive technique and also because more instruments are now commercially available from various manufacturers. The ECS technique is currently used to monitor the post-illumination decay of the proton-motive force (pmf), but it has a more general use for quantitative studies on photosynthetic energy transduction. Here we briefly summarize the basic knowledge on ECS, emphasize the full potential of this technique, and propose a quantitative analysis of the photosynthetic performance with the definition of a transmission coefficient for electrons along the photosynthetic chain. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Availability: |
https://hal.science/hal-03368441; https://hal.science/hal-03368441v1/document; https://hal.science/hal-03368441v1/file/Standard%20units%20for%20ElectroChromic%20Shift%20accepted%20modifications.pdf |
| Rights: |
https://about.hal.science/hal-authorisation-v1/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.50FAAEC |
| Database: |
BASE |