| Title: |
Home-HIT improves muscle capillarisation and eNOS/NAD(P)Hoxidase protein ratio in obese individuals with elevated cardiovascular disease risk. |
| Authors: |
Scott, SN; Shepherd, SO; Hopkins, ND; Dawson, EA; Strauss, JA; Wright, DJ; Cooper, RG; Kumar, P; Wagenmakers, AJM; Cocks, MS |
| Publisher Information: |
Wiley |
| Publication Year: |
2019 |
| Collection: |
Liverpool John Moores University: LJMU Research Online |
| Subject Terms: |
RC1200 Sports Medicine |
| Description: |
KEY POINTS: Obesity and sedentary behaviour are associated with capillary rarefaction and impaired muscle microvascular vasoreactivity, due to reduced nitric oxide bioavailability. Low-volume high-intensity interval training (HIT) is a time-efficient alternative to traditional moderate-intensity continuous training (MICT), but its effect on the muscle microvasculature has not been studied. The applicability of current lab- and gym-based HIT protocols for obese individuals with low fitness and mobility has been disputed by public health experts, who cite the strenuous nature and complex protocols as major barriers. Therefore, we developed a virtually-supervised HIT protocol targeting this group that can be performed at home without equipment (Home-HIT). This study is the first to show that 12-weeks of virtually-supervised Home-HIT in obese individuals with elevated CVD risk leads to similar increases in capillarisation and eNOS/NAD(P)Hoxidase protein ratio within the muscle microvascular endothelium as virtually-supervised home-based MICT and laboratory-based HIT, while reducing many of the major barriers to exercise. ABSTRACT: This study investigated the effect of a novel virtually-supervised home-based high-intensity interval training (HIT) (Home-HT) intervention in obese individuals with elevated cardiovascular disease (CVD) risk on capillarisation and muscle microvascular eNOS/NAD(P)Hoxidase ratio. Thirty-two adults with elevated CVD risk (age 36 ± 10 years; BMI 34.3 ± 5 kg∙m-2 ; V̇O2peak 24.6 ± 5.7 ml∙kg∙min-1 ), completed one of three 12-week training programmes: Home-HIT (n = 9); laboratory-based supervised HIT (Lab-HIT; n = 10) or virtually-supervised home-based moderate-intensity continuous training (Home-MICT; n = 13). Muscle biopsies were taken pre- and post-training to assess changes in vascular enzymes, capillarisation, mitochondrial density, intramuscular triglyceride content and GLUT4 protein expression using quantitative immunofluorescence microscopy. Training increased V̇O2peak (P < 0.001), ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
text |
| Language: |
English |
| ISSN: |
1469-7793 |
| Relation: |
https://researchonline.ljmu.ac.uk/id/eprint/10949/1/ScottJP278062%282019%29.pdf; Scott, SN, Shepherd, SO, Hopkins, ND, Dawson, EA, Strauss, JA, Wright, DJ, Cooper, RG, Kumar, P, Wagenmakers, AJM and Cocks, MS (2019) Home-HIT improves muscle capillarisation and eNOS/NAD(P)Hoxidase protein ratio in obese individuals with elevated cardiovascular disease risk. Journal of Physiology. ISSN 1469-7793 |
| DOI: |
10.1113/JP278062 |
| Availability: |
https://researchonline.ljmu.ac.uk/id/eprint/10949/; https://researchonline.ljmu.ac.uk/id/eprint/10949/1/ScottJP278062%282019%29.pdf; https://doi.org/10.1113/JP278062 |
| Accession Number: |
edsbas.518EEF80 |
| Database: |
BASE |