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Biohydrogen and short chain fatty acids production by dark fermentation from agri-food residues

Title: Biohydrogen and short chain fatty acids production by dark fermentation from agri-food residues
Authors: Guez, Jean-Sébastien; Fontanille, Pierre; Fleitas García, Ana-Rosa; El Abyad, Sara; Christophe, Gwendoline; Vignigbe, Emeric Candide; Dubessay, Pascal; Vial, Christophe; Taha, Samir
Contributors: Institut Pascal (IP); SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne 2017-2020 (UCA 2017-2020 )-Centre National de la Recherche Scientifique (CNRS); Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne); Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA); Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-SIGMA Clermont (SIGMA Clermont)-Centre National de la Recherche Scientifique (CNRS); Procédés Biologiques, Génie Enzymatique et Microbien - EA1026 (ProBioGEM); Université de Lille, Sciences et Technologies; Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne); Université Clermont Auvergne (UCA); Génie des Procédés, Energétique et Biosystèmes (GePEB); SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne 2017-2020 (UCA 2017-2020 )-Centre National de la Recherche Scientifique (CNRS)-SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne 2017-2020 (UCA 2017-2020 )-Centre National de la Recherche Scientifique (CNRS); Laboratoire de Génie Chimique et Biochimique (LGCB); Université Blaise Pascal - Clermont-Ferrand 2 (UBP); Clermont Auvergne Métropole CAM; European Project: 722361,H2020-NMBP-2016-2017,H2020-NMBP-ERA-NET-2016,ERA CoBioTech(2016)
Source: Société Française de Microbiologie SFM 2025 ; https://hal.science/hal-05172700 ; Société Française de Microbiologie SFM 2025, Sep 2025, Bordeaux, France
Publisher Information: CCSD
Publication Year: 2025
Collection: HAL Clermont Auvergne (Université Blaise Pascal Clermont-Ferrand / Université d'Auvergne)
Subject Terms: microbial population analysis; Biomass valorisation; short chain fatty acid; biohydrogen; anaerobic fermentation; [SDV.BIO]Life Sciences [q-bio]/Biotechnology; [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry; Molecular Biology/Biochemistry [q-bio.BM]; [SDV.EE.IEO]Life Sciences [q-bio]/Ecology; environment/Symbiosis; [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology; [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering
Subject Geographic: Bordeaux; France
Description: International audience ; In the ongoing energy transition, hydrogen (H2) has emerged as a promising energy carrier due to its high calorific value and the fact that its combustion produces only water. By now, fossil fuel-based technologies remain more cost-effective, with H2 production costs around 0.8-2.8 € kg-1H2 via steam methane reforming (Amin et al., 2022). But numerous ongoing efforts aim to develop more cleaner and still competitive H2production methods, including water-electrolysis, water splitting, thermo-chemical and biological processes (Agyekum et al., 2022). Considering biological process, they have been explored with more interest in recent years. The so-called dark fermentation (DF) is the acidogenic stage of anaerobic digestion in which bacteria convert organic matter into bioH2 and small chain volatile fatty acids (SCFA), in the absence of light. Numerous efforts to study these processes were done on a laboratory scale, but there still is a lack of operational and scalable data, to provide references for future industrial operations.The main objective of this work was to explore critical process parameters having an effect on the production of bioH2 during DF from real substrates in stirred tank reactor (STR). During the intensification of process, SCFAs were also inspected, as they can potentially be transformed to bioH2 in a second stage, under conditions exposed to light, which allow to improve the overall substrate conversion efficiency.An original result of the study was to assess the impact of the initial redox on H₂ production. We sought to identify the optimal range of initial redox that improves H₂ production and yield using both a model substrate (Fleitas et al., 2024) and real ones, as depackaging food wastes (Fleitas et al., 2025) or whey, within experiments done in STR. The second objective was to examine the influence of redox control on H₂ production. To achieve this objective, the determination of a redox range that allow to maximize H₂ production was obtained by actively ...
Document Type: conference object
Language: English
Relation: info:eu-repo/grantAgreement//722361/EU/Cofund on Biotechnologies/ERA CoBioTech
Availability: https://hal.science/hal-05172700
Rights: http://creativecommons.org/licenses/by-nc/
Accession Number: edsbas.5297661F
Database: BASE