| Title: |
Choline Modulates Soluble‐Endoglin (sEng) and Vascular Endothelial Growth Factor (VEGF) in Cultured Human Placental Trophoblasts |
| Authors: |
Andrew, Benjamin Yates; Jiang, Xinyin; Jones, Sara; Caudill, Marie A; Brannon, Patsy M |
| Contributors: |
U.S. Department of Agriculture; National Institute of Food and Agriculture |
| Source: |
The FASEB Journal ; volume 27, issue S1 ; ISSN 0892-6638 1530-6860 |
| Publisher Information: |
Wiley |
| Publication Year: |
2013 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Altering the balance between the anti‐angiogenic factors, soluble fms‐related tyrosine kinase‐1 (sFLT1) and sEng, and the angiogenic factors, VEGF and transforming growth factor beta (TGF‐β), contributes to preeclampsia (PE) and persistence of hypoxia (Hx). Increasing sFLT1 and sEng may sequester VEGF and TGF‐β. Recently we showed that supplemental choline reduced placental sFLT1 mRNA and maternal plasma sFLT1 in pregnant women and cultured trophoblasts (HTR‐8/SVneo). To determine if choline regulates sEng production and VEGF gene expression independently or interactively with Hx, HTR‐8/SVneo cells were cultured in a 2×4 factorial design in either normoxia (20% O 2 , Nx) or Hx (1% O 2 ) and 13 μM, 18 μM, 28 μM (control) or 48 μM for 96 hours. Results were analyzed by 2‐way ANOVA (SigmaPlot). Low choline (13 μM) under Nx, but not Hx, increased media sEng (14%, p=0.036) and mRNA levels of VEGF (160.9%, p |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1096/fasebj.27.1_supplement.1077.7 |
| Availability: |
http://dx.doi.org/10.1096/fasebj.27.1_supplement.1077.7 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.52F1E8F7 |
| Database: |
BASE |