| Title: |
Anti-Inflammatory Effects of Honeysuckle Leaf Against Lipopolysaccharide-Induced Neuroinflammation on BV2 Microglia |
| Authors: |
Bitna Kweon; Jinyoung Oh; Yebin Lim; Gyeongran Noh; Jihyun Yu; Donggu Kim; Mikyung Jang; Donguk Kim; Gisang Bae |
| Source: |
Nutrients ; Volume 16 ; Issue 22 ; Pages: 3954 |
| Publisher Information: |
Multidisciplinary Digital Publishing Institute |
| Publication Year: |
2024 |
| Collection: |
MDPI Open Access Publishing |
| Subject Terms: |
Lonicera japonica Thunb; leaf of honeysuckle (LH); honeysuckle; neuroinflammation; microglia |
| Subject Geographic: |
agris |
| Description: |
Background/Objectives: Neurodegenerative disorders have emerged as a major global public health concern, and the burden is predicted to increase over time. Modulating neuroinflammation and microglial activity is considered a promising target for improving neurodegenerative disorders. The leaf of honeysuckle (LH), which has anti-inflammatory properties, has long been collected, regardless of the season, and used for medicinal purposes. However, research on its effects on neuroinflammation is scarce. In this study, we determined the neuroprotective effects of LH water extract by inhibiting microglial activation induced by lipopolysaccharide (LPS). Methods: The production or secretion of pro-inflammatory mediators was examined in LPS-exposed BV2 cells to ascertain the efficacy of LH water extract in improving neuroinflammation. In addition, the phosphorylation of mitogen-activated protein kinases (MAPKs) and the degradation of inhibitory κBα (IκBα) were analyzed to elucidate the regulatory mechanisms of LH water extract. Ultra-performance liquid chromatography (UPLC) analysis was conducted to identify the active component of the LH. Results: LH water extract suppressed the formation of inducible nitric oxide synthase (iNOS), nitric oxide (NO), and pro-inflammatory cytokines, including interleukin (IL)-1β and tumor necrosis factor (TNF)-α, in LPS-activated BV2 cells. LH impeded the activation of c-Jun N-terminal kinase (JNK). Moreover, chlorogenic acid was found in LH. Conclusions: The above findings suggest that LH water extract could improve neuroinflammation. |
| Document Type: |
text |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
Phytochemicals and Human Health; https://dx.doi.org/10.3390/nu16223954 |
| DOI: |
10.3390/nu16223954 |
| Availability: |
https://doi.org/10.3390/nu16223954 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.53EB3A04 |
| Database: |
BASE |