| Title: |
Quantification of surface GalNAc ligands decorating nanostructured lipid carriers by UPLC-ELSD |
| Authors: |
Gauthier, Laura; Varache, Mathieu; Couffin, Anne-Claude; Lebrun, Colette; Delangle, Pascale; Gateau, Christelle; Texier, Isabelle |
| Contributors: |
Chimie Interface Biologie pour l’Environnement, la Santé et la Toxicologie (CIBEST); SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SYMMES); Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 )-Institut de Recherche Interdisciplinaire de Grenoble (IRIG); Direction de Recherche Fondamentale (CEA) (DRF (CEA)); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 )-Institut de Recherche Interdisciplinaire de Grenoble (IRIG); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA); Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI); Direction de Recherche Technologique (CEA) (DRT (CEA)); Université Grenoble Alpes (UGA); ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017) |
| Source: |
ISSN: 1661-6596. |
| Publisher Information: |
CCSD; MDPI |
| Publication Year: |
2019 |
| Collection: |
Université Grenoble Alpes: HAL |
| Subject Terms: |
nanostructured lipid carriers; N-acetyl-D-galactosamine ligand; surface functionalization; ligand quantification; [SPI]Engineering Sciences [physics]; [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics |
| Description: |
International audience ; Nanoparticles have been extensively studied for drug delivery and targeting to specific organs. The functionalization of the nanoparticle surface by site-specific ligands (antibodies, peptides, saccharides) can ensure efficient recognition and binding with relevant biological targets. One of the main challenges in the development of these decorated nanocarriers is the accurate quantification of the amount of ligands on the nanoparticle surface. In this study, nanostructured lipid carriers (NLC) were functionalized with N-acetyl-D-galactosamine (GalNAc) units, known to target the asialoglycoprotein receptor (ASGPR). Different molar percentages of GalNAc-functionalized surfactant (0%, 2%, 5%, and 14%) were used in the formulation. Based on ultra-high-performance liquid chromatography separation and evaporative light-scattering detection (UPLC-ELSD), an analytical method was developed to specifically quantify the amount of GalNAc units present at the NLC surface. This method allowed the accurate quantification of GalNAc surfactant and therefore gave some insights into the structural parameters of these multivalent ligand systems. Our data show that the GalNAc decorated NLC possess large numbers of ligands at their surface and suitable distances between them for efficient multivalent interaction with the ASGPR, and therefore promising liver-targeting efficiency. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.3390/ijms20225669 |
| Availability: |
https://cea.hal.science/cea-04783480; https://cea.hal.science/cea-04783480v1/document; https://cea.hal.science/cea-04783480v1/file/ijms-20-05669-v2.pdf; https://doi.org/10.3390/ijms20225669 |
| Rights: |
http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.54B3AEFC |
| Database: |
BASE |