| Title: |
Quantification of Cardiomyocyte Hypertrophy by Cardiac Magnetic Resonance ; Implications for Early Cardiac Remodeling |
| Authors: |
Coelho-Filho, Otavio R.; Shah, Ravi V.; Mitchell, Richard; Neilan, Tomas G.; Moreno, Heitor; Simonson, Bridget; Kwong, Raymond; Rosenzweig, Anthony; Das, Saumya; Jerosch-Herold, Michael |
| Source: |
Circulation ; volume 128, issue 11, page 1225-1233 ; ISSN 0009-7322 1524-4539 |
| Publisher Information: |
Ovid Technologies (Wolters Kluwer Health) |
| Publication Year: |
2013 |
| Description: |
Background— Cardiomyocyte hypertrophy is a critical precursor to the development of heart failure. Methods to phenotype cellular hypertrophy noninvasively are limited. The goal was to validate a cardiac magnetic resonance–based approach for the combined assessment of extracellular matrix expansion and cardiomyocyte hypertrophy. Methods and Results— Two murine models of hypertension (n=18, with n=15 controls) induced by l - N G -nitroarginine methyl ester (L-NAME) and pressure overload (n=11) from transaortic constriction (TAC) were imaged by cardiac magnetic resonance at baseline and 7 weeks after L-NAME treatment or up to 7 weeks after TAC. T1 relaxation times were measured before and after gadolinium contrast. The intracellular lifetime of water ( τ ic ), a cell size–dependent parameter, and extracellular volume fraction, a marker of interstitial fibrosis, were determined with a model for transcytolemmal water exchange. Cardiomyocyte diameter and length were measured on FITC–wheat germ agglutinin–stained sections. The τ ic correlated strongly with histological cardiomyocyte volume-to-surface ratio ( r =0.78, P |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1161/circulationaha.112.000438 |
| DOI: |
10.1161/CIRCULATIONAHA.112.000438 |
| Availability: |
https://doi.org/10.1161/circulationaha.112.000438; https://www.ahajournals.org/doi/full/10.1161/CIRCULATIONAHA.112.000438 |
| Accession Number: |
edsbas.55AFC78E |
| Database: |
BASE |