| Title: |
A cancer immunoprofiling strategy using mass spectrometry coupled with bioorthogonal cleavage |
| Authors: |
Ribéraud, Maxime; Porret, Estelle; Pruvost, Alain; Theodoro, Frédéric; Nguyen, Anvi, Laëtitia; Specklin, Simon; Kereselidze, Dimitri; Denis, Caroline; Jego, Benoit; Barbe, Peggy; Keck, Mathilde; d'Anfray, Timothée; Kuhnast, Bertrand; Audisio, Davide; Truillet, Charles; Taran, Frédéric |
| Contributors: |
Médicaments et Technologies pour la Santé (MTS); Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); LaBoratoire d'Imagerie biOmédicale MultimodAle Paris-Saclay (BIOMAPS); Service Hospitalier Frédéric Joliot (SHFJ); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Source: |
ISSN: 2041-6520. |
| Publisher Information: |
CCSD; The Royal Society of Chemistry |
| Publication Year: |
2024 |
| Subject Terms: |
[SDV.CAN]Life Sciences [q-bio]/Cancer |
| Description: |
International audience ; The accurate quantification of biomarkers is paramount in modern medicine, particularly in cancer where precise diagnosis is imperative for targeted therapy selection. In this paper we described a multiplexed analysis diagnostic approach based on cleavable MS-tagged antibodies. The technology uses MS-tag isotopologues and the sydnonimine-cyclooctyne click-and-release bioorthogonal reaction. In a proof of concept study, we demonstrated the potential of this approach for cancer cell immunoprofiling in culture cells, tissues and in vivo as well, thereby unveiling promising diagnostic avenues. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/39464609; PUBMED: 39464609; WOS: 001338552700001 |
| DOI: |
10.1039/d4sc04471a |
| Availability: |
https://hal.science/hal-05032431; https://hal.science/hal-05032431v1/document; https://hal.science/hal-05032431v1/file/d4sc04471a.pdf; https://doi.org/10.1039/d4sc04471a |
| Rights: |
http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.55B5878F |
| Database: |
BASE |