| Title: |
Prophylactic intranasal administration of a TLR2/6 agonist reduces upper respiratory tract viral shedding in a SARS-CoV-2 challenge ferret model |
| Authors: |
Proud, PC; Tsitoura, D; Watson, RJ; Chua, BY; Aram, MJ; Bewley, KR; Cavell, BE; Cobb, R; Dowall, S; Fotheringham, SA; Ho, CMK; Lucas, V; Ngabo, D; Rayner, E; Ryan, KA; Slack, GS; Thomas, S; Wand, NI; Yeates, P; Demaison, C; Zeng, W; Holmes, I; Jackson, DC; Bartlett, NW; Mercuri, F; Carroll, MW |
| Publisher Information: |
ELSEVIER |
| Publication Year: |
2021 |
| Collection: |
The University of Melbourne: Digital Repository |
| Description: |
BACKGROUND: The novel human coronavirus SARS-CoV-2 is a major ongoing global threat with huge economic burden. Like all respiratory viruses, SARS-CoV-2 initiates infection in the upper respiratory tract (URT). Infected individuals are often asymptomatic, yet highly infectious and readily transmit virus. A therapy that restricts initial replication in the URT has the potential to prevent progression of severe lower respiratory tract disease as well as limiting person-to-person transmission. METHODS: SARS-CoV-2 Victoria/01/2020 was passaged in Vero/hSLAM cells and virus titre determined by plaque assay. Challenge virus was delivered by intranasal instillation to female ferrets at 5.0 × 106 pfu/ml. Treatment groups received intranasal INNA-051, developed by Ena Respiratory. SARS-CoV-2 RNA was detected using the 2019-nCoV CDC RUO Kit and QuantStudio™ 7 Flex Real-Time PCR System. Histopathological analysis was performed using cut tissues stained with haematoxylin and eosin (H&E). FINDINGS: We show that prophylactic intra-nasal administration of the TLR2/6 agonist INNA-051 in a SARS-CoV-2 ferret infection model effectively reduces levels of viral RNA in the nose and throat. After 5 days post-exposure to SARS-CoV-2, INNA-051 significantly reduced virus in throat swabs (p= |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| ISSN: |
2352-3964 |
| Relation: |
https://hdl.handle.net/11343/272186 |
| Availability: |
https://hdl.handle.net/11343/272186 |
| Rights: |
https://creativecommons.org/licenses/by-nc-nd/4.0 ; CC BY-NC-ND |
| Accession Number: |
edsbas.563156D8 |
| Database: |
BASE |