| Title: |
The effect of dietary red palm oil on the functional recovery of the ischaemic/reperfused isolated rat heart: the involvement of the PI3-Kinase signaling pathway. |
| Authors: |
Engelbrecht, AM; Odendaal, L; Du Toit, EF; Kupai, K; Csont, T; Ferdinandy, P; Van Rooyen, J |
| Publisher Information: |
BioMed Central |
| Publication Year: |
2009 |
| Collection: |
Griffith University: Griffith Research Online |
| Subject Terms: |
Other information and computing sciences; Medical biochemistry and metabolomics; Cardiology (incl. cardiovascular diseases); Nutrition and dietetics; Medical physiology not elsewhere classified |
| Description: |
We have previously shown that dietary red palm oil (RPO) supplementation improves functional recovery in hearts subjected to ischaemia/reperfusion-induced injury. Unfortunately, the cellular and molecular mechanisms responsible for this phenomenon are still poorly understood and no knowledge exists regarding the effects of RPO supplementation on the phosphoinositide 3-kinase (PI3-K) signaling pathway and apoptosis during ischaemia/reperfusion injury. Therefore, the aims of the present study were three fold: (i) to establish the effect of RPO on the functional recovery of the heart after ischaemia/reperfuion injury; (ii) to determine the effect of the PI3-K pathway in RPO-induced protection with the aid of an inhibitor (wortmannin); and (iii) to evaluate apoptosis in our model. Wistar rats were fed a standard rat chow control diet or a control diet plus 7 g RPO/kg for six weeks. Hearts were excised and mounted on a Langendorff perfusion apparatus. Mechanical function was measured after a 25 min period of total global ischaemia followed by 30 minutes of reperfusion. Hearts subjected to the same conditions were freeze-clamped for biochemical analysis at 10 min during reperfusion to determine the involvement of the PI3-Kinase signaling pathway and apoptosis in our model. Dietary RPO supplementation significantly increased % rate pressure product recovery during reperfusion (71.0 ᠶ.3% in control vs 92.36 ᠴ.489% in RPO; p < 0.05). The % rate pressure product recovery was significantly reduced when wortmannin was added during perfusion (92.36 ᠴ.489% in the RPO group vs 75.21 ᠵ.26% in RPO + Wm). RPO + Wm also significantly attenuated PI3-K induction compared with the RPO group (59.2 ᠲ.8 pixels in RPO vs 37.9 ᠳ.4 pixels in RPO + Wm). We have also demonstrated that PI3-K inhibition induced PARP cleavage (marker of apoptosis) in the hearts during ischaemia/reperfusion injury and that RPO supplementation counteracted this effect. ; Full Text |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
Lipids in Health and Disease; https://hdl.handle.net/10072/33956 |
| DOI: |
10.1186/1476-511X-8-18 |
| Availability: |
https://hdl.handle.net/10072/33956; https://doi.org/10.1186/1476-511X-8-18 |
| Rights: |
http://creativecommons.org/licenses/by/2.0 ; © 2009 Engelbrecht et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ; open access |
| Accession Number: |
edsbas.56837954 |
| Database: |
BASE |