| Title: |
Radiation-induced extracellular matrix remodelling drives prognosis and predicts radiotherapy response in muscle-invasive bladder cancer. |
| Authors: |
Guerrero Quiles, C; Fahy, S; Bartak, M; Gonzalez Abalos, J; Powell, E; Lodhi, T; Reed, R; Reeves, K; Baker, A; James, ND; Hall, E; Huddart, RA; Porta, N; Hoskin, P; West, C; Biolatti, LV; Choudhury, A |
| Contributors: |
James, Nicholas; Hall, Emma; Huddart, Robert; Porta, Nuria |
| Publisher Information: |
FRONTIERS MEDIA SA |
| Publication Year: |
2025 |
| Collection: |
The Institute of Cancer Research (ICR): Publications Repository |
| Subject Terms: |
ECM - extracellular matrix; biomarker; bladder cancer (BCa); radioresistance mechanisms; radiotherapy |
| Subject Geographic: |
Switzerland |
| Description: |
Muscle-invasive bladder cancer (MIBC) is a prevalent disease that can be treated with radiotherapy, but has a poor prognosis. Radiation-induced extracellular matrix (ECM) remodelling and fibrosis can induce tumour resistance and recurrence, but have not been studied in MIBC. Here, we aimed to characterise the impact of radiation on the ECM composition of MIBC. Three MIBC cell lines (T24, UMUC3, J82) were treated with fractionated radiation. We used proteomics to analyse the ECM composition produced by surviving cancer cells and immunofluorescence to investigate changes in the morphology and number of ECM fibres. We evaluated the RNA expression of identified ECM proteins (FN1, COL5A1, COL1A1, TNF6AIP6, FLG) in one cystectomy (TCGA-BLCA, n=397) and two radiotherapy (BC2001, n=313; BCON, n=151) cohorts. There were 613 proteins affected by radiation (padj2 or |
| Document Type: |
article in journal/newspaper |
| File Description: |
Electronic-eCollection; application/pdf |
| Language: |
English |
| ISSN: |
2234-943X |
| Relation: |
1616943; Frontiers in Oncology, 2025, 15 pp. 1616943 -; https://repository.icr.ac.uk/handle/internal/6775 |
| DOI: |
10.3389/fonc.2025.1616943 |
| Availability: |
https://doi.org/10.3389/fonc.2025.1616943; https://repository.icr.ac.uk/handle/internal/6775 |
| Rights: |
http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.57170C93 |
| Database: |
BASE |