| Title: |
Nerve growth factor induces proliferation of PC12 cells through Cdc42 |
| Authors: |
Seo, JY; Kim, JS; Ghang, JH; Kang, TC; Suh, JG; Kim, EG; Kim, JI; Kim, J; Lee, JY; Park, JB |
| Contributors: |
Kim, JI |
| Publisher Information: |
Lippincott Williams & Wilkins Ltd. |
| Publication Year: |
2025 |
| Collection: |
Seoul National University: S-Space |
| Subject Terms: |
CYCLIN D1 EXPRESSION; NEURITE OUTGROWTH; PROTEIN-KINASE; MAP KINASE; RHOA; ACTIVATION; INDUCTION; Cdc42; cell proliferation; cyclin D1; nerve growth factor; Rho family GTPase; p21(WAF1); PC12 cell |
| Description: |
Nerve growth factor (NGF) can change neurite outgrowth and cellular morphology of rat adrenal pheochromocytorna PC 12 cells. In the present study, stable PC 12 cells with constitutively active form (L6ICdc42), the dominant negative constructs (N17Cdc42), and wild type Cdc42 did not proliferate in low serum condition. However, on exposure to NGF, PC 12 cells transfected with L61Cdc42 cDNA proliferated, whereas wild type Cdc42 and N17Cdc42 cDNA-transfected PC12 cells did not. When the cells were stimulated with NGF, ERK1/2 was transiently activated in L61Cdc42-transfected PC12 cells, whereas the activation of ERK1/2 was sustained in other cell lines. NGF repressed the induction of p21(WAF1), cyclin-dependent kinase (CDK) inhibitor, in L61Cdc42 transfected PC12 cells, whereas NGF induced the expression of p21(WAF1) in other PC12 cell lines. ; N ; 1 |
| Document Type: |
article in journal/newspaper |
| Language: |
unknown |
| Relation: |
https://hdl.handle.net/10371/225313; 000184486400018; 240468 |
| DOI: |
10.1097/00001756-200307010-00018 |
| Availability: |
https://hdl.handle.net/10371/225313; https://doi.org/10.1097/00001756-200307010-00018 |
| Accession Number: |
edsbas.5787AB |
| Database: |
BASE |