| Title: |
Search of a genomic sequence database for potential novel blood group antigens: Investigation into why some amino acid substitutions are not immunogenic |
| Authors: |
Howe, John G.; Stack, Gary |
| Source: |
Transfusion ; volume 63, issue 7, page 1399-1411 ; ISSN 0041-1132 1537-2995 |
| Publisher Information: |
Wiley |
| Publication Year: |
2023 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Background Polypeptide blood group antigens are typically identified through investigation of the antibodies they induce. Human genome sequence databases are a new tool to identify AA substitutions that potentially create blood group antigens. Study Design and Methods The Erythrogene genomic sequence database was searched for missense mutations not known to be blood group antigens in the extracellular domains of selected RBC proteins in European populations. Any mutations found with prevalence of 1%–90% and not known to have induced antibodies in transfusion practice were analyzed using protein structural analysis and epitope prediction programs to determine why they apparently lack immunogenicity. Results Thirteen missense mutations not known to create blood group antigens were identified in the extracellular domains of Kell, BCAM, and RhD proteins, but not in RhCE, Urea Transporter 1 (Kidd), Atypical Chemokine Receptor 1 (Duffy), glycophorin A or glycophorin B. While 11 of the 13 mutations had low prevalence ( |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1111/trf.17459 |
| Availability: |
https://doi.org/10.1111/trf.17459; https://onlinelibrary.wiley.com/doi/pdf/10.1111/trf.17459 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.587111F |
| Database: |
BASE |