| Title: |
Remodeling of the Actin Network Associated with the Non-Structural Protein 1 (NS1) of West Nile Virus and Formation of NS1-Containing Tunneling Nanotubes |
| Authors: |
Furnon, Wilhelm; Fender, Pascal; Confort, Marie-Pierre; Desloire, Sophie; Nangola, Sawitree; Kitidee, Kuntida; Leroux, Caroline; Ratinier, Maxime; Arnaud, Frederick; Lecollinet, Sylvie; Boulanger, Pierre; Hong, Saw-See |
| Contributors: |
Infections Virales et Pathologie Comparée - UMR 754 (IVPC); Institut National de la Recherche Agronomique (INRA)-École Pratique des Hautes Études (EPHE); Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Université de Lyon; Institut de biologie structurale (IBS - UMR 5075); Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 )-Institut de Recherche Interdisciplinaire de Grenoble (IRIG); Direction de Recherche Fondamentale (CEA) (DRF (CEA)); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA); Department of Medical Technology, School of Allied Health Sciences; Center for Research & Innovation; Mahidol University Bangkok; Virologie UMR1161 (VIRO); École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES); Institut Francais du Cheval et de l'Equitation (IFCE Grant) Equine Virus Tools-2017 WNV_ARELIA-2018French Cystic Fibrosis Association (VLM Grant) RF2016 050 1671French National Research Agency (ANR) 18-CE11-0001French Ministery of Foreign Affairs PHC SIAM 2017-2018; ANR-18-CE11-0001,Ad-Cadh,Mécanisme d'interaction des adenovirus du sous-groupe B2 à la cadhérine desmosomale DSG2 et ses applications(2018) |
| Source: |
ISSN: 1999-4915 ; Viruses ; https://hal.science/hal-02361065 ; Viruses, 2019, 11 (10), pp.901. ⟨10.3390/v11100901⟩. |
| Publisher Information: |
CCSD; MDPI |
| Publication Year: |
2019 |
| Collection: |
HAL Lyon 1 (University Claude Bernard Lyon 1) |
| Subject Terms: |
tunneling nanotubes; non-structural protein 1 (NS1); West Nile virus; actin network; cytoskeleton; flavivirus; [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry; Molecular Biology/Structural Biology [q-bio.BM] |
| Description: |
International audience ; The cellular response to the recombinant NS1 protein of West Nile virus (NS1WNV) was studied using three different cell types: Vero E6 simian epithelial cells, SH-SY5Y human neuroblastoma cells, and U-87MG human astrocytoma cells. Cells were exposed to two different forms of NS1WNV: (i) the exogenous secreted form, sNS1WNV, added to the extracellular milieu; and (ii) the endogenous NS1WNV, the intracellular form expressed in plasmid-transfected cells. The cell attachment and uptake of sNS1WNV varied with the cell type and were only detectable in Vero E6 and SH-SY5Y cells. Addition of sNS1WNV to the cell culture medium resulted in significant remodeling of the actin filament network in Vero E6 cells. This effect was not observed in SH-SY5Y and U-87MG cells, implying that the cellular uptake of sNS1WNV and actin network remodeling were dependent on cell type. In the three cell types, NS1WNV-expressing cells formed filamentous projections reminiscent of tunneling nanotubes (TNTs). These TNT-like projections were found to contain actin and NS1WNV proteins. Interestingly, similar actin-rich, TNT-like filaments containing NS1WNV and the viral envelope glycoprotein EWNV were also observed in WNV-infected Vero E6 cells. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/31569658; PRODINRA: 495586; PUBMED: 31569658; WOS: 000498400400022 |
| DOI: |
10.3390/v11100901 |
| Availability: |
https://hal.science/hal-02361065; https://hal.science/hal-02361065v1/document; https://hal.science/hal-02361065v1/file/2019_Furnon_Viruses_1.pdf; https://doi.org/10.3390/v11100901 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.589FCC52 |
| Database: |
BASE |