| Title: |
ELIME-IMS hybrid assay for Salmonella detection in swine mesenteric lymph nodes at slaughterhouse |
| Authors: |
Trevisani M.; Conter M.; Cecchini M.; Lamperti L.; Andriani L.; Rega M.; Bacci C.; Perri M.; Bonardi S. |
| Contributors: |
Trevisani, M.; Conter, M.; Cecchini, M.; Lamperti, L.; Andriani, L.; Rega, M.; Bacci, C.; Perri, M.; Bonardi, S. |
| Publication Year: |
2025 |
| Collection: |
IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
| Subject Terms: |
Carcasse; ISO 6579–1:2017; Salmonella; Screening test; Slaughterhouse; electrochemical biosensors. immunomagnetic bead; lymph nodes |
| Description: |
Salmonella contamination in pig slaughterhouses is linked to infection rate on farms. Accurate diagnosis in heavy pigs relies on isolating pathogens from the gut wall or lymph nodes. A key technique is Immunocapture using Magnetic Beads (IMS), which purifies target bacteria from Salmonella enrichment broths. This is followed by an Enzyme-Linked Immunomagnetic Electrochemical (ELIME) assay for rapid detection. In our study, we developed an ELIME-IMS hybrid assay to detect Salmonella in swine mesenteric lymph nodes (MNL), involving a clean-up with N-acetylcysteine and centrifugation. Detection limits for S. Typhimurium and S. Derby were estimated at 2.80 and 3.52 Log CFU/ml, respectively. We analysed 103 MNL samples from a northern Italy slaughterhouse. Additionally, we examined 15 carcass swabs. Both the ELIME assay and the IMS-based culture method showed strong agreement with the ISO 6579-1:2017 method, especially after 20 h of enrichment (89.47% concordance). The clean-up step significantly influenced the results, as samples processed without it showed higher variability. A logistic regression model indicated high classification accuracy for negative samples using ELIME values. The ELIME-IMS assay facilitates rapid Salmonella screening and isolation in swine mesenteric lymph nodes. |
| Document Type: |
article in journal/newspaper |
| File Description: |
ELETTRONICO |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/39448169; info:eu-repo/semantics/altIdentifier/wos/WOS:001340891700001; volume:125; firstpage:104659; lastpage:104668; numberofpages:10; journal:FOOD MICROBIOLOGY; https://hdl.handle.net/11585/997687 |
| DOI: |
10.1016/j.fm.2024.104659 |
| Availability: |
https://hdl.handle.net/11585/997687; https://doi.org/10.1016/j.fm.2024.104659; https://www.sciencedirect.com/science/article/pii/S0740002024001977?ref=pdf_download&fr=RR-2&rr=8ea35d4a0c99ea41 |
| Rights: |
info:eu-repo/semantics/openAccess ; license:Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY) ; license uri:iris.PUB15 |
| Accession Number: |
edsbas.58FFC4B5 |
| Database: |
BASE |