| Title: |
Development of a molecular aptamer beacon applied to magnetic-assisted RNA extraction for detection of dengue and zika viruses using clinical samples |
| Authors: |
da Silva, Amanda Gabrielle; Goulart, Luiz Ricardo; Löffler, Philipp; Code, Christian; Neves, Adriana Freitas |
| Source: |
da Silva, A G, Goulart, L R, Löffler, P, Code, C & Neves, A F 2022, 'Development of a molecular aptamer beacon applied to magnetic-assisted RNA extraction for detection of dengue and zika viruses using clinical samples', International Journal of Molecular Sciences, vol. 23, no. 22, 13866. https://doi.org/10.3390/ijms232213866 |
| Publication Year: |
2022 |
| Collection: |
University of Southern Denmark: Research Output / Syddansk Universitet |
| Subject Terms: |
diagnosis; flavivirus; hybridization; oligonucleotides; RT-PCR |
| Description: |
Limitations in the detection of cocirculating flaviviruses such as Dengue and Zika lead us to propose the use of aptameric capture of the viral RNA in combination with RT-PCR (APTA-RT-PCR). Aptamers were obtained via SELEX and next-generation sequencing, followed by colorimetric and fluorescent characterizations. An APTA-RT-PCR assay was developed, optimized, and tested against the viral RNAs in 108 serum samples. After selection, sequence APTAZC10 was designed as a bifunctional molecular beacon (APTAZC10-MB), exhibiting affinity for the viral targets. APTA-RT-PCR was able to detect Dengue and Zika RNA in 43% and 8% of samples, respectively. Our results indicate that APTAZC10-MB and APTA-RT-PCR will be useful to improve the detection of Dengue and Zika viruses in a fast molecular assay for the improvement of infectious disease surveillance. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| ISSN: |
1422-0067 |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/36430340; info:eu-repo/semantics/altIdentifier/pissn/1422-0067 |
| DOI: |
10.3390/ijms232213866 |
| Availability: |
https://portal.findresearcher.sdu.dk/da/publications/2a9fa866-9fd7-4e9c-9221-f7ccd2f0e342; https://doi.org/10.3390/ijms232213866; https://findresearcher.sdu.dk/ws/files/215612247/ijms_23_13866.pdf |
| Rights: |
info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.596997A3 |
| Database: |
BASE |