| Title: |
Human COQ10A and COQ10B are distinct lipid-binding START domain proteins required for coenzyme Q function[S] |
| Authors: |
Tsui, Hui S; Pham, Nguyen VB; Amer, Brendan R; Bradley, Michelle C; Gosschalk, Jason E; Gallagher-Jones, Marcus; Ibarra, Hope; Clubb, Robert T; Blaby-Haas, Crysten E; Clarke, Catherine F |
| Source: |
Journal of Lipid Research, vol 60, iss 7 |
| Publisher Information: |
eScholarship, University of California |
| Publication Year: |
2019 |
| Collection: |
University of California: eScholarship |
| Subject Terms: |
3101 Biochemistry and Cell Biology (for-2020); 31 Biological Sciences (for-2020); Complementary and Integrative Health (rcdc); Genetics (rcdc); Dietary Supplements (rcdc); Nutrition (rcdc); 1.1 Normal biological development and functioning (hrcs-rac); Antioxidants (mesh); Ataxia (mesh); Humans (mesh); Lipid Peroxidation (mesh); Mass Spectrometry (mesh); Mitochondria (mesh); Mitochondrial Diseases (mesh); Muscle Weakness (mesh); Oxidative Stress (mesh); Phosphoproteins (mesh); Saccharomyces cerevisiae (mesh); Saccharomyces cerevisiae Proteins (mesh); Ubiquinone (mesh); Steroidogenic Acute Regulatory Protein (mesh); antioxidants; lipids; chemistry; peroxidation; mass spectrometry; mitochondria; Saccharomyces cerevisiae; ubiquinone; steroidogenic acute regulatory protein-related lipid transfer |
| Time: |
1293 - 1310 |
| Description: |
Coenzyme Q (CoQ or ubiquinone) serves as an essential redox-active lipid in respiratory electron and proton transport during cellular energy metabolism. CoQ also functions as a membrane-localized antioxidant protecting cells against lipid peroxidation. CoQ deficiency is associated with multiple human diseases; CoQ10 supplementation in particular has noted cardioprotective benefits. In Saccharomyces cerevisiae, Coq10, a putative START domain protein, is believed to chaperone CoQ to sites where it functions. Yeast coq10 deletion mutants (coq10Δ) synthesize CoQ inefficiently during log phase growth and are respiratory defective and sensitive to oxidative stress. Humans have two orthologs of yeast COQ10, COQ10A and COQ10B Here, we tested the human co-orthologs for their ability to rescue the yeast mutant. We showed that expression of either human ortholog, COQ10A or COQ10B, rescues yeast coq10Δ mutant phenotypes, restoring the function of respiratory-dependent growth on a nonfermentable carbon source and sensitivity to oxidative stress induced by treatment with PUFAs. These effects indicate a strong functional conservation of Coq10 across different organisms. However, neither COQ10A nor COQ10B restored CoQ biosynthesis when expressed in the yeast coq10Δ mutant. The involvement of yeast Coq10 in CoQ biosynthesis may rely on its interactions with another protein, possibly Coq11, which is not found in humans. Coexpression analyses of yeast COQ10 and human COQ10A and COQ10B provide additional insights to functions of these START domain proteins and their potential roles in other biologic pathways. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
unknown |
| Relation: |
qt3qx9m140; https://escholarship.org/uc/item/3qx9m140; https://escholarship.org/content/qt3qx9m140/qt3qx9m140.pdf |
| DOI: |
10.1194/jlr.m093534 |
| Availability: |
https://escholarship.org/uc/item/3qx9m140; https://escholarship.org/content/qt3qx9m140/qt3qx9m140.pdf; https://doi.org/10.1194/jlr.m093534 |
| Rights: |
CC-BY |
| Accession Number: |
edsbas.59FDDF9B |
| Database: |
BASE |