| Title: |
A short report on melanocyte/melanoma culture, senescence, and reproducibility |
| Authors: |
Larue, Lionel; Barral, Duarte, C; Delmas, Veronique; Egea-Rodriguez, Sara; Aldea, Daniel; Etchevers, Heather, C; Galibert, Marie-Dominique; Kelsh, Robert, N; Lanfrancone, Luisa; Madigan, Michele; Moura-Alves, Pedro; White, Richard, M; Bosserhoff, Anja |
| Contributors: |
Signalisation, radiobiologie et cancer; Institut Curie Paris -Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS); Universidade Nova de Lisboa = NOVA University Lisbon (NOVA); Ludwig-Maximilians University Munich (LMU); University Hospital LMU Munich; Ludwig Maximilian University Munich = Ludwig Maximilians Universität München (LMU); Marseille medical genetics - Centre de génétique médicale de Marseille (MMG); Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM); Institut Marseille Maladies Rares (MarMaRa); Aix Marseille Université (AMU); Institut de Génétique et Développement de Rennes (IGDR); Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes (Biosit : Biologie - Santé - Innovation Technologique); Biologie et génétique du cancer; Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS); Centre Hospitalier Universitaire de Rennes CHU Rennes = Rennes University Hospital Pontchaillou; University of Bath Bath; European Institute of Oncology Milan (ESMO); University of New South Wales Sydney (UNSW); Universidade do Porto = University of Porto; University of Oxford; Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg (FAU); None |
| Source: |
ISSN: 1755-1471 ; Pigment Cell and Melanoma Research ; https://amu.hal.science/hal-05533385 ; Pigment Cell and Melanoma Research, 2026, 39 (2), pp.e70084. ⟨10.1111/pcmr.70084⟩. |
| Publisher Information: |
CCSD |
| Publication Year: |
2026 |
| Subject Terms: |
[SDV]Life Sciences [q-bio]; [SDV.BC]Life Sciences [q-bio]/Cellular Biology |
| Description: |
International audience ; At the 2025 ESPCR (European Society for Pigment Cell Research) meeting in Erlangen, a workshop on "Pigment Cell Models: Sensitivity, Innovation, and the Challenges of Cell Culture" brought together researchers to discuss technical, methodological, and reproducibility issues in culturing melanocytes, keratinocytes, fibroblasts, and melanoma cells. The discussion between experts in the field highlighted key and recurrent pitfalls affecting experimental outcomes, including low-density seeding, temperature fluctuations, over-passaging, and mycoplasma contamination, as well as sources of variability arising from media composition, batch effects, and environmental conditions. Importantly, the workshop distinguished between practices supported by evidence and consensus-based guidance derived from collective expert experience. Species- and donor-specific differences, especially between human, mouse, and zebrafish melanocyte models, were identified as additional major determinants of experimental variability. Emerging systems, including human and mouse pluripotent stem cell (PSC)-derived melanocytes, as well as avian and zebrafish melanoma lines, were discussed for their complementary mechanistic and translational value. Overall, the workshop concluded that transparent documentation, explicit reporting standards, and shared best practices are essential to improve reproducibility and further advance pigment cell research. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/41807282; PUBMED: 41807282; PUBMEDCENTRAL: PMC12975704 |
| DOI: |
10.1111/pcmr.70084 |
| Availability: |
https://amu.hal.science/hal-05533385; https://amu.hal.science/hal-05533385v3/document; https://amu.hal.science/hal-05533385v3/file/Pigment%20Cell%20Melanoma%20Res%20-%202026%20-%20Larue%20-%20A%20Short%20Report%20on%20Melanocyte%20Melanoma%20Culture%20%20Senescence%20%20and%20Reproducibility.pdf; https://doi.org/10.1111/pcmr.70084 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.5A2FC8AF |
| Database: |
BASE |