| Title: |
Reducing ischemic kidney injury through application of a synchronization modulation electric field to maintain Na + /K + -ATPase functions |
| Authors: |
Chen, Wei; Wang, Lei; Liang, Pengfei; Mast, Jason; Mathis, Clausell; Liu, Catherine Y.; Wei, Jin; Zhang, Jie; Fu, Liying; Juncos, Luis A.; Buggs, Jacentha; Liu, Ruisheng |
| Source: |
Science Translational Medicine ; volume 14, issue 635 ; ISSN 1946-6234 1946-6242 |
| Publisher Information: |
American Association for the Advancement of Science (AAAS) |
| Publication Year: |
2022 |
| Description: |
Renal ischemia-reperfusion injury is an important contributor to the development of delayed graft function after transplantation, which is associated with higher rejection rates and poorer long-term outcomes. One of the earliest impairments during ischemia is Na + /K + -ATPase (Na/K pump) dysfunction due to insufficient ATP supply, resulting in subsequent cellular damage. Therefore, strategies that preserve ATP or maintain Na/K pump function may limit the extent of renal injury during ischemia-reperfusion. Here, we applied a synchronization modulation electric field to activate Na/K pumps, thereby maintaining cellular functions under ATP-insufficient conditions. We tested the effectiveness of this technique in two models of ischemic renal injury: an in situ renal ischemia-reperfusion injury model (predominantly warm ischemia) and a kidney transplantation model (predominantly cold ischemia). Application of the synchronization modulation electric field to a renal ischemia-reperfusion injury mouse model preserved Na/K pump activity, thereby reducing kidney injury, as reflected by 40% lower plasma creatinine (1.17 ± 0.03 mg/dl) in the electric field–treated group as compared to the untreated control group (1.89 ± 0.06 mg/dl). In a mouse kidney transplantation model, renal graft function was improved by more than 50% with the application of the synchronization modulation electric field according to glomerular filtration rate measurements (85.40 ± 12.18 μl/min in the untreated group versus 142.80 ± 11.65 μl/min in the electric field–treated group). This technique for preserving Na/K pump function may have therapeutic potential not only for ischemic kidney injury but also for other diseases associated with Na/K pump dysfunction due to inadequate ATP supply. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1126/scitranslmed.abj4906 |
| Availability: |
https://doi.org/10.1126/scitranslmed.abj4906; https://www.science.org/doi/pdf/10.1126/scitranslmed.abj4906 |
| Accession Number: |
edsbas.5AA5FBFF |
| Database: |
BASE |