| Title: |
RT-PCR confirmations of the vCircTrappist results. |
| Authors: |
Alexis S. Chasseur; Maxime Bellefroid; Mathilde Galais; Meijiao Gong; Pierre Lombard; Sarah Mathieu; Amandine Pecquet; Estelle Plant; Camille Ponsard; Laure Vreux; Carlo Yague-Sanz; Benjamin G. Dewals; Nicolas A. Gillet; Benoît Muylkens; Carine M. Van Lint; Damien Coupeau |
| Publication Year: |
2025 |
| Subject Terms: |
Medicine; Microbiology; Genetics; Molecular Biology; Evolutionary Biology; Immunology; Infectious Diseases; Virology; Biological Sciences not elsewhere classified; +non%22">xlink "> non; unconventional splicing mechanisms; sanger sequencings validated; recent attention focused; obtained novel insights; initially anticipated due; identify backsplicing events; human pathogens belonging; existing datasets encompassing; conserved eukaryotic patterns; coding rnas play; viral infection cycles; viral genomes include; unique viral characteristics; rna sequencing data; developed bioinformatic tool; various viral families; bioinformatic pipeline tailored; families |
| Description: |
(A) Viral circRNAs expressed from an in vivo infection with AlHV-1. A lymphoblastoid cell line (LCL) from in vivo -infected calves was recovered and grown in culture. (B-C-D) RT-PCR confirmations of the loci of circRNA expression in the AlHV-1 model. Untreated samples and corresponding linear RNAs were used as controls for the RNase R treatment. White arrows were placed at the expected band size to facilitate reading. (E) Viral circRNAs expressed from an in vitro infection with MDV. The ESCDL-1 cell line was infected with the RB-1B strain of the virus and the RNAs were recovered after 6 days of infection. (F-G) RT-PCR confirmations of the loci of circRNA expression in the MDV model. Untreated samples and corresponding linear RNAs were used as controls for the RNase R treatment. White arrows were placed at the expected band size to facilitate reading. (H) Viral circRNAs expressed from an in vitro culture of productively HTLV-1-infected cells (SLB1 cell line). (I-J) RT-PCR confirmations of the loci of circRNA expression in the HTLV-1 model. Untreated samples and corresponding linear RNAs were used as controls for the RNase R treatment. The second sample represents a false positive result. White arrows were placed at the expected band size to facilitate reading. (K) Viral circRNAs expressed from BLV productively-infected cell line L267 LTaxSN . (L-M) RT-PCR confirmations of the loci of circRNA expression in the BLV model. Untreated samples and corresponding linear RNAs were used as controls for the RNase R treatment. White arrows were placed at the expected band size to facilitate reading. (N) Viral circRNA identification after 24h of an infection with the HAdV-C5. The infection was performed using A549 cells. (O-P) RT-PCR confirmations of the loci of circRNA expression in the HAdV-C5 model. Untreated samples and corresponding linear RNAs were used as controls for the RNase R treatment. White arrows were placed at the expected band size to facilitate reading. (A-E-H-N) Relevant ORFs were indicated under the graphs ... |
| Document Type: |
still image |
| Language: |
unknown |
| DOI: |
10.1371/journal.ppat.1013448.s007 |
| Availability: |
https://doi.org/10.1371/journal.ppat.1013448.s007; https://figshare.com/articles/figure/RT-PCR_confirmations_of_the_vCircTrappist_results_/30105326 |
| Rights: |
CC BY 4.0 |
| Accession Number: |
edsbas.5B7C2D2D |
| Database: |
BASE |