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CD8 + Skin-Resident Memory T Cells Require TCR Signaling for their Persistence in a Mouse Model of Allergic Contact Dermatitis

Title: CD8 + Skin-Resident Memory T Cells Require TCR Signaling for their Persistence in a Mouse Model of Allergic Contact Dermatitis
Authors: Funch, Anders Boutrup; Weber, Julie Friis; Mraz, Veronika; Kongsbak-Wismann, Martin; Lohmann, Rebecca Kitt Davidson; Jee, Mia Hamilton; Vaher, Helen; Yeung, Kelvin; Gadsbøll, Anne Sofie Østergaard; Ødum, Niels; Woetmann, Anders; Johansen, Jeanne Duus; Geisler, Carsten; Bonefeld, Charlotte Menné
Source: Funch , A B , Weber , J F , Mraz , V , Kongsbak-Wismann , M , Lohmann , R K D , Jee , M H , Vaher , H , Yeung , K , Gadsbøll , A S Ø , Ødum , N , Woetmann , A , Johansen , J D , Geisler , C & Bonefeld , C M 2026 , ' CD8 + Skin-Resident Memory T Cells Require TCR Signaling for their Persistence in a Mouse Model of Allergic Contact Dermatitis ' , Journal of Investigative Dermatology , vol. 146 , no. 1 ....
Publication Year: 2026
Collection: University of Copenhagen: Research / Forskning ved Københavns Universitet
Subject Terms: Allergic contact dermatitis; Epidermis; Nur77; TCR; Tissue-resident memory T cells
Description: CD8 + epidermal-resident memory T (T RM ) cells play a significant role in fighting off pathogens. However, CD8 + T RM cells are also central in the pathogenesis of a variety of inflammatory skin diseases. It is unclear whether the generation and persistence of CD8 + T RM cells are dependent on the presence of cognate antigen and TCR signaling. The purpose of this study was to determine whether TCR signaling is required for the generation and persistence of epidermal CD8 + T RM cells in a mouse model for allergic contact dermatitis. We examined the responses to 4 different contact allergens in combination with adoptive transfer and prime-pull experiments. We determined the presence of contact allergen in the skin by western blot analysis. We found that epidermal CD8 + T RM cells can develop in the absence of the cognate antigen and TCR signaling as determined by Nur77 induction, whereas persistence of epidermal CD8 + T RM cells requires the presence of the cognate antigen and correlates with Nur77 expression. In the presence of contact allergen, a selective expansion of specific TCR clonotypes was seen. In conclusion, this study supports that cognate antigen and TCR signaling are required for the persistence of allergen-specific CD8 + T RM cells in the skin.
Document Type: article in journal/newspaper
File Description: application/pdf
Language: English
DOI: 10.1016/j.jid.2025.05.012
Availability: https://researchprofiles.ku.dk/da/publications/dc34b074-250f-4441-a75d-1529b013baea; https://doi.org/10.1016/j.jid.2025.05.012; https://curis.ku.dk/ws/files/534175670/CD8.pdf
Rights: info:eu-repo/semantics/openAccess
Accession Number: edsbas.5B87690A
Database: BASE