| Title: |
Development of a synthetic mating-responsive promoter. |
| Authors: |
Sandrine Pinheiro; Mariona Nadal-Ribelles; Carme Solé; Vincent Vincenzetti; Yves Dusserre; Francesc Posas; Serge Pelet |
| Publication Year: |
2025 |
| Collection: |
The University of Auckland: Figshare |
| Subject Terms: |
Microbiology; Cell Biology; Genetics; Molecular Biology; Immunology; Developmental Biology; Science Policy; Infectious Diseases; Plant Biology; Biological Sciences not elsewhere classified; two haploid cells; saccharomyces cerevisiae < p; core promoter modulate; ste12 binding sites; binding sites; strong dimer; specific timing; largely encoded; large diversity; basal association |
| Description: |
A. Scheme of the various promoter configurations tested starting from the p AGA1 endogenous reporter and exchanging the core promoter and testing regulatory regions with a UAS containing various configurations of PREs. B. Dynamics of nuclear enrichment for the dPSTR R under the control of various synthetic promoters. The colored solid lines represent the median of the population and the shaded area, the 25- and 75-percentile of the population. The solid black line is the reference induction from the endogenous promoter p AGA1 and the dashed line is the control promoter without PRE sites. C. Fraction of strongly (dark bar) and weakly (light bar) responding cells relative to the p AGA1 -dPSTR R . The total fraction of responding cells from individual replicates is displayed by the markers. D. Histogram of the difference in response time between the tested promoter and the internal reference provided by the p AGA1 -dPSTR Y . E. Dynamics of nuclear enrichment for the p AGA1 -dPSTR Y (left) and p SYN -dPSTR R (right) variants in WT (solid line) and ste12∆ (dashed lines) strains. F. Fraction of responding cells in the WT and ste12∆ strains for the p AGA1 -dPSTR Y (left) and p SYN -dPSTR R (right) variants. (PDF) |
| Document Type: |
article in journal/newspaper |
| Language: |
unknown |
| DOI: |
10.1371/journal.pgen.1011710.s003 |
| Availability: |
https://doi.org/10.1371/journal.pgen.1011710.s003; https://figshare.com/articles/journal_contribution/Development_of_a_synthetic_mating-responsive_promoter_/29332252 |
| Rights: |
CC BY 4.0 |
| Accession Number: |
edsbas.5BD3B31B |
| Database: |
BASE |