| Title: |
Optimizing in vitro micropropagation strategies in Santalum album L. through different explants |
| Authors: |
Gowsalya, M; Jude, S R; Hemaprabha, K; Revathi, R; Mathivanan, M; Parthiban, K T; Sekar, I; Kiruba, M; Sujith, P P; Kabinesh, V |
| Source: |
Plant Science Today; Vol. 12 No. sp1 (2025): Recent Advances in Agriculture by Young Minds - II ; 2348-1900 ; 10.14719/pst.2025.12.sp1 |
| Publisher Information: |
Horizon e-Publishing Group |
| Publication Year: |
2025 |
| Collection: |
Horizon e-Publishing Group (HePG): E-Journals |
| Subject Terms: |
Indian sandalwood; explants disinfection; micropropagation; plant growth regulators; shoot induction |
| Description: |
This study focuses on optimization of in vitro propagation protocols for Santalum album L. (Indian sandalwood) to address the increasing global demand and to aid in conservation aspects. This investigation evaluated various micropropagation parameters including explant types, basal media, disinfection methods and plant growth regulators (PGR). Murashige and Skoog medium consistently outperformed other media, with shoot tips showing the highest morphogenetic response (66.66%). Surface disinfection with 4% NaOCl for 10 min was the most effective and resulted in the explant survival rate of 86%. For shoot induction, 2 mg/L kinetin in MS medium resulted in the highest number of shoots (3.81) and longest shoots (5.39 cm) after 60 days. An effect was observed when PGR was combined with 5.0 mg/L kinetin + 2.0 mg/L BAP, corresponding to a shoot induction rate of 65.75%. Root induction was recorded at 14.79% after 10 days with the best treatment (MS + 1 mg/L IBA). Callus culture showed limited success as only one treatment (MS + 1 mg/L BAP + 25% coconut water) showed a regenerative response of 7.14%. These results provide a foundation for micropropagation of Sandal while highlighting areas that require further optimization, particularly in root induction and acclimation phases. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
https://horizonepublishing.com/journals/index.php/PST/article/view/5991/5414; https://horizonepublishing.com/journals/index.php/PST/article/view/5991/5415; https://horizonepublishing.com/journals/index.php/PST/article/view/5991 |
| Availability: |
https://horizonepublishing.com/journals/index.php/PST/article/view/5991 |
| Rights: |
Copyright (c) 2025 Gowsalya M, Jude Sudhagar R, Hemaprabha K, Revathi R, Mathivanan M, Parthiban K T, Sekar I, Kiruba M, Sujith P P, Kabinesh V ; https://creativecommons.org/licenses/by/4.0 |
| Accession Number: |
edsbas.5C761BCB |
| Database: |
BASE |