| Title: |
Cytoskeletal Regulation Couples LFA-1 Conformational Changes to Receptor Lateral Mobility and Clustering |
| Authors: |
Christopher W. Cairo; Rossen Mirchev; David E. Golan |
| Contributors: |
The Pennsylvania State University CiteSeerX Archives |
| Source: |
http://www.chem.ualberta.ca/~cairo/pubs/immunity2006.s1.pdf. |
| Collection: |
CiteSeerX |
| Subject Terms: |
Section S5. Deconvolution of Subpopulations |
| Description: |
Particle trajectories were analyzed using an MSD analysis, as described by Saxton and Jacobson. 1 High-speed video frames (1000 FPS) were analyzed using a patternmatching algorithm implemented in Metamorph (Universal Imaging). The trajectory was then read into a custom MATlab program and converted to a mean square displacement (MSD) vs. time interval curve. MSD was calculated as the average displacement of a given time interval averaged over all increments. The MSD vs. time interval curve was then analyzed to determine microdiffusion (Dmicro) and macrodiffusion (Dmacro) coefficients. Section S2. Microdiffusion Fit Micro-diffusion coefficients were calculated by performing a linear fit of the first four increments of the MSD vs. time interval curve (4 ms). Therefore, the slope of the fit represented the average diffusion coefficient over this short time interval. Fit values are given in the accompanying spreadsheet. Section S3. Macrodiffusion Fit Macro-diffusion coefficients were calculated by fitting the initial third of the MSD vs. time interval curve to the following equation: 2 α < r> = 4Dt (1) Here, is the MSD, t is the time interval, D is the time-dependent diffusion coefficient (Dmacro), and the α coefficient classifies the mode of anomalous diffusion. 2 Fit values with α < 0.7 are considered to represent confined or corralled motion, values with 0.7 < α < 1.2 are consistent with brownian diffusion, and values with α> 1.2 are considered to represent flow diffusion. 1 Fit values are given in the accompanying spreadsheet. |
| Document Type: |
text |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.324.4343; http://www.chem.ualberta.ca/~cairo/pubs/immunity2006.s1.pdf |
| Availability: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.324.4343; http://www.chem.ualberta.ca/~cairo/pubs/immunity2006.s1.pdf |
| Rights: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| Accession Number: |
edsbas.5D84018A |
| Database: |
BASE |