| Title: |
High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications |
| Authors: |
Alter, Claudio Luca; Detampel, Pascal; Schefer, Roman Basil; Lotter, Claudia; Hauswirth, Patrick; Puligilla, Ramya Deepthi; Weibel, Vera Jo; Schenk, Susanne Heidi; Heusermann, Wolf; Schürz, Melanie; Meisner-Kober, Nicole; Palivan, Cornelia; Einfalt, Tomaz; Huwyler, Jörg |
| Publisher Information: |
Zenodo |
| Publication Year: |
2023 |
| Collection: |
Zenodo |
| Subject Terms: |
exosome; apoptotic vesicles; plasma membrane vesiculation; phosphatidylserine; in vivo distribution |
| Description: |
Extracellular vesicles (EVs) are highly interesting for the design of next-generation therapeutics. However, their preparation methods face challenges in standardization, yield, and reproducibility. Here, we describe a highly efficient and reproducible EV preparation method for monodisperse nano plasma membrane vesicles (nPMVs), which yields 10 to 100 times more particles per cell and hour than conventional EV preparation methods. nPMVs are produced by homogenizing giant plasma membrane vesicles following cell membrane blebbing and apoptotic body secretion induced by chemical stressors. nPMVs showed no significant differences compared to native EVs from the same cell line in cryo-TEM analysis, in vitro cellular interactions, and in vivo biodistribution studies in zebrafish larvae. Proteomics and lipidomics, on the other hand, suggested substantial differences consistent with the divergent origin of these two EV types and indicated that nPMVs primarily derive from apoptotic extracellular vesicles. nPMVs may provide an attractive source for developing EV‑based pharmaceutical therapeutics. ; We gratefully acknowledge the financial support provided by the Swiss Nanoscience Institute (SNI-P1801), the Swiss National Science Foundation (CRSII5_180257), the Phospholipid Research Center Heidelberg (JHU-2021-094/2-1), and Swiss Centre for Applied Human Toxicology – SCAHT (SCAHT-GL 21-09). |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://zenodo.org/records/7817772; oai:zenodo.org:7817772; https://doi.org/10.5281/zenodo.7817772 |
| DOI: |
10.5281/zenodo.7817772 |
| Availability: |
https://doi.org/10.5281/zenodo.7817772; https://zenodo.org/records/7817772 |
| Rights: |
Creative Commons Attribution 4.0 International ; cc-by-4.0 ; https://creativecommons.org/licenses/by/4.0/legalcode |
| Accession Number: |
edsbas.5DF74C91 |
| Database: |
BASE |