| Title: |
Mass spectrometry‐based absolute protein quantification: PSAQ™ strategy makes use of “noncanonical” proteotypic peptides |
| Authors: |
Jaquinod, Michel; Trauchessec, Mathieu; Huillet, Céline; Louwagie, Mathilde; Lebert, Dorothée; Picard, Guillaume; Adrait, Annie; Dupuis, Alain; Garin, Jérôme; Brun, Virginie; Bruley, Christophe |
| Contributors: |
CEA Life Sciences division, the INSERM, the Joseph Fourier University, the French Association pour la Recherche sur le Cancer, the French National Agency; 7th Framework Programme of the European Union |
| Source: |
PROTEOMICS ; volume 12, issue 8, page 1217-1221 ; ISSN 1615-9853 1615-9861 |
| Publisher Information: |
Wiley |
| Publication Year: |
2012 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Absolute quantification of proteins using isotope dilution mass spectrometry requires the selection of proteotypic peptides. When choosing these peptides, a certain number of rules must be respected. Several of these were established to safeguard against quantification errors resulting from the isotopically labeled standard peptides not behaving in the same way as the peptides to be quantified. Of all absolute quantification methods using isotope dilution, P rotein S tandard for A bsolute Q uantification ( PSAQ TM ) offers the maximal protein sequence coverage. In the present study, we show that the PSAQ method presents a previously unreported advantage for protein quantification as it makes use of M et/ C ys‐containing peptides and peptides‐containing miscleavages in addition to proteotypic peptides. By increasing the total number of peptides that can be considered, robustness of quantification is improved, paving the way for a facilitated quantification of low abundant and/or low‐molecular‐weight proteins. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1002/pmic.201100538 |
| DOI: |
10.1002/pmic.201100538/fullpdf |
| Availability: |
https://doi.org/10.1002/pmic.201100538; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fpmic.201100538; http://onlinelibrary.wiley.com/wol1/doi/10.1002/pmic.201100538/fullpdf |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.5E324D95 |
| Database: |
BASE |