| Title: |
Formin Is Associated with Left-Right Asymmetry in the Pond Snail and the Frog |
| Authors: |
Davison, Angus; McDowell, Gary S.; Holden, Jennifer M.; Johnson, Harriet F.; Koutsovoulos, Georgios D.; Liu, M. Maureen; Hulpiau, Paco; van Roy, Frans; Wade, Christopher M.; Banerjee, Ruby; Yang, F.; Chiba, Satoshi; Davey, John W.; Jackson, Daniel John; Levin, Michael; Blaxter, Mark L. |
| Contributors: |
Davison, Angus; McDowell, Gary S.; Holden, Jennifer M.; Johnson, Harriet F.; Koutsovoulos, Georgios D.; Liu, M. Maureen; Hulpiau, Paco; van Roy, Frans; Wade, Christopher M.; Banerjee, Ruby; Yang, F.; Chiba, Satoshi; Davey, John W.; Jackson, Daniel John; Levin, Michael; Blaxter, Mark L. |
| Publisher Information: |
Cell Press |
| Publication Year: |
2016 |
| Collection: |
Georg-August-Universität Göttingen: GoeScholar |
| Description: |
While components of the pathway that establishes left-right asymmetry have been identified in diverse animals, from vertebrates to flies, it is striking that the genes involved in the first symmetry-breaking step remain wholly unknown in the most obviously chiral animals, the gastropod snails. Previously, research on snails was used to show that left-right signaling of Nodal, downstream of symmetry breaking, may be an ancestral feature of the Bilateria [1, 2]. Here, we report that a disabling mutation in one copy of a tandemly duplicated, diaphanous-related formin is perfectly associated with symmetry breaking in the pond snail. This is supported by the observation that an anti-formin drug treatment converts dextral snail embryos to a sinistral phenocopy, and in frogs, drug inhibition or overexpression by microinjection of formin has a chirality-randomizing effect in early (pre-cilia) embryos. Contrary to expectations based on existingmodels [3-5], we discovered asymmetric gene expression in 2- and 4-cell snail embryos, preceding morphological asymmetry. As the formin-actin filament has been shown to be part of an asymmetry-breaking switch in vitro [6, 7], together these results are consistent with the view that animals with diverse body plans may derive their asymmetries from the same intracellular chiral elements [8]. |
| Document Type: |
article in journal/newspaper |
| Language: |
unknown |
| ISBN: |
978-0-00-371401-2; 0-00-371401-2 |
| Relation: |
000371401200024 |
| DOI: |
10.1016/j.cub.2015.12.071 |
| Availability: |
https://resolver.sub.uni-goettingen.de/purl?gro-2/41134; https://resolver.sub.uni-goettingen.de/purl?gs-1/14154; https://doi.org/10.1016/j.cub.2015.12.071 |
| Rights: |
info:eu-repo/semantics/openAccess ; CC BY 4.0 ; https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.5E35C918 |
| Database: |
BASE |